An acetylcholinesterase gene, acel, has been cloned and sequenced from both susceptible and field strains of Culex pipiens pallens. Comparison of both nucleic acid and deduced amino acid sequences revealed an amino acid substitution. The mutation A328S corresponding to A201S in Torpedo californica abutting the oxyanion hole of AChE1, might affect the stability of enzyme-substrate transition state and result in insecticide resistance. The mutation had been found in seven field strains of Cx. pipiens pallens collected in Beijing, China, and also had been detected in the Labagoumen strain by PCR-restriction endonuclease assay during 2003–2005. We suggested that A201S replacement in the acel gene is possibly responsible for insensitivity of AChE1 to some carbamates or organophosphates in field strains of mosquitoes.
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1 December 2009
An Amino Acid Substitution on the Acetylcholinesterase in the Field Strains of House Mosquito, Culex Pipiens Pallens(Diptera: Culicidae) in China
Chun-Xiao Li,
Yan-De Dong,
Feng-Lin Song,
Xiao-Long Zhang,
Tong-Yan Zhao
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acetylcholinesterase
China
Culex pipiens pallens
Culicidae
Diptera
mutation
target site
