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1 December 2013 A Simple and Rapid Dna Extraction Protocol of Small Insects for Pcr Amplification
En-Wei Tian, Hui Yu
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A procedure to extract genomic DNA from small insects of Blastophaga javana Hill (Hymenoptera: Agaoninae) and Ceratosolen solmsi marchali (Hymenoptera: Agaoninae) for PCR amplification is described. We compared the amplification of both cytochrome oxidase I (COI) gene and nuclear gene (Microsatellites) for specimens with different storage times in 95% ethanol. We found that with the modified protocol, a single, clean DNA fragment from the specimens can be amplified. The PCR amplifications from specimens with shorter storage times are much better. We found that the fragment that the microsatellite (SSR) amplified from B. javana is single and stable. This method is simple, reliable, economical, needs minimal equipment and reagents, and is important in addressing the difficulty of DNA extraction from a single very small insect.

En-Wei Tian and Hui Yu "A Simple and Rapid Dna Extraction Protocol of Small Insects for Pcr Amplification," Entomological News 123(4), 303-310, (1 December 2013).
Received: 3 August 2012; Accepted: 20 August 2013; Published: 1 December 2013
Blastophata Javana
Ceratosolen solmsi
COI amplification
DNA extraction
small insects
SSR amplification
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