It has been shown that honey bees (Apis mellifera L.) adsorb bacterial spores as a function of the electrostatic charge on the bee and concentration of bacteria in the aerosol during tethered flight in wind tunnel experiments. This report presents a mathematical model for predicting the number of spores that could be adsorbed onto free-flying bees passing through a bacterial spore aerosol plume/cloud and experimental validation of this model. The model accounts for the geometry of the aerosol dispersion from a continuous point source, aerosol particle settling, and adsorption and desorption rates onto/off of bees based on laboratory observations of tethered flying honey bees.