European corn borer females Ostrinia nubilalis Hübner (Lepidoptera, Pyralidae) exhibited upwind flight to three main host plants, corn Zea mays, hemp Cannabis sativa and hop Humulus lupulus, in a laboratory wind tunnel. Within a 15-min experimental period, 22.9% to 24.3% mated females flew toward and landed on a single potted corn plant. A potted hemp plant attracted 25.7% females. In a choice test, significantly more females landed on a hemp plant than on an adjacent corn plant. In contrast, paprika Capsicum annuum did not elicit attraction. Headspace collections from corn, hemp, and hop contained 18 compounds which consistently elicited a response from female antennae. Four of these, (Z)-3-hexenyl acetate, β-caryophyllene, (E)-β-farnesene, and (E,E)-α-farnesene co-occurred in three host plants studied. A 4-component blend of these compounds did not attract female moths in the wind tunnel. Availability of a wind tunnel bioassay is, however, a step toward the identification of plant volatiles guiding long-range attraction of gravid corn borer females.
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