We examined the nematode fauna associated with a species of bark beetle, Dryocoetes uniseriatus Eggers, as part of a biodiversity survey of forest beetle-associated nematodes. Collections were made in a pine stand at an experimental forest station in Ibaraki, Japan, from April to July of 2011; we examined the nematode association in 273 insects collected during this time. In total, 68% of the insects were associated with at least one species of nematode. Six species of nematodes, including two phoretic microbe feeders (Bursaphelenchus rainulfi Braasch & Burgermeister and Micoletzkya sp.), one insect parasite and nematode predator (Devibursaphelenchus cf. eproctatus), one insect parasite (Contortyhnchus sp.), one insect parasite and potential microbe feeder (unidentified rhabditid parasite), and one potential insect parasite and fungal feeder (B. sinensis) were recovered from the beetles. D. cf. eproctatus was enclosed in nematangia on the backsides of the elytra, B. rainulfi was isolated from the backsides of the elytra or enclosed in nematangia, Micoletzkya sp. was isolated from under the elytra, Contortylenchus sp. and a rhabditid parasite parasitized the body cavity, and B. sinensis was found in the digestive tract of the insect. The association patterns of the nematode species varied seasonally, although definitive interactions among species (e.g., segregation, competition) were not observed.