Translator Disclaimer
22 September 2015 Essential Amino Acid Supplementation by Gut Microbes of a Wood-Feeding Cerambycid
Paul A. Ayayee, Thomas Larsen, Cristina Rosa, Gary W. Felton, James G. Ferry, Kelli Hoover
Author Affiliations +
Abstract

Insects are unable to synthesize essential amino acids (EAAs) de novo, thus rely on dietary or symbiotic sources for them. Wood is a poor resource of nitrogen in general, and EAAs in particular. In this study, we investigated whether gut microbiota of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), a cerambycid that feeds in the heartwood of healthy host trees, serve as sources of EAAs to their host under different dietary conditions. δ13C-stable isotope analyses revealed significant δ13C-enrichment (3.4 ± 0.1%; mean ± SEM) across five EAAs in wood-fed larvae relative to their woody diet. δ13C values for the consumers greater than 1% indicate significant contributions from non-dietary EAA sources (symbionts in this case). In contrast, δ13Cenrichment of artificial diet-fed larvae (controls) relative to their food source was markedly less (1.7 ± 0.1%) than was observed in wood-fed larvae, yet still exceeded the threshold of 1%. A predictive model based on δ13Ceaa signatures of five EAAs from representative bacterial, fungal, and plant samples identified symbiotic bacteria and fungi as the likely supplementary sources of EAA in wood-fed larvae. Using the same model, but with an artificial diet as the dietary source, we identified minor supplementary bacterial sources of EAA in artificial diet-fed larvae. This study highlights how microbes associated with A. glabripennis can serve as a source of EAAs when fed on nutrient-limited diets, potentially circumventing the dietary limitations of feeding on woody substrates.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Paul A. Ayayee, Thomas Larsen, Cristina Rosa, Gary W. Felton, James G. Ferry, and Kelli Hoover "Essential Amino Acid Supplementation by Gut Microbes of a Wood-Feeding Cerambycid," Environmental Entomology 45(1), 66-73, (22 September 2015). https://doi.org/10.1093/ee/nvv153
Received: 17 May 2015; Accepted: 28 August 2015; Published: 22 September 2015
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top