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8 December 2015 Diet-Delivered dsRNAs for Juvenile Hormone-Binding Protein and Vacuolar ATPase-H Implied Their Potential in the Management of the Melon Aphid (Hemiptera: Aphididae)
K. B. Rebijith, R. Asokan, H. H. Ranjitha, B. S. Rajendra Prasad, V. Krishna, N. K. Krishna Kumar
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Abstract

RNA interference is a sequence-specific gene silencing mechanism mediated by double-stranded RNA (dsRNA), which has been harnessed as a useful tool in devising novel insect pest management strategies for various pests such as melon aphid, Aphis gossypii (Glover). In the current study, we cloned and sequenced juvenile hormone-binding protein (JHBP) and vacuolar ATPase subunit H (V-ATPase-H) from A. gossypii. We also showed the effectiveness of diet-mediated delivery of dsRNA for JHBP and V-ATPase-H, which silenced the above genes and resulted in mortality. The extent of silencing and mortality were similar for both genes up until 96 h. Bioassay results revealed that the target genes were silenced variably, 1.0 μg/μl concentration having a more profound effect than 0.5 and 0.25 μg/μl concentration in reducing the cognate mRNA transcript level. Results indicated a 9.56–73.21% down regulation (across time and concentrations for both the genes) that resulted in the mortality of A. gossypii. Mortality was in the range of 10–63% for both these genes. Thus, the current study demonstrated the potentiality of both JHBP and V-ATPase-H as excellent targets for the management of A. gossypii.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
K. B. Rebijith, R. Asokan, H. H. Ranjitha, B. S. Rajendra Prasad, V. Krishna, and N. K. Krishna Kumar "Diet-Delivered dsRNAs for Juvenile Hormone-Binding Protein and Vacuolar ATPase-H Implied Their Potential in the Management of the Melon Aphid (Hemiptera: Aphididae)," Environmental Entomology 45(1), 268-275, (8 December 2015). https://doi.org/10.1093/ee/nvv178
Received: 5 August 2015; Accepted: 4 November 2015; Published: 8 December 2015
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