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23 September 2017 Rapid Cold-Hardening of a Subtropical Species, Maruca vitrata (Lepidoptera: Crambidae), Accompanies Hypertrehalosemia by Upregulating Trehalose-6-Phosphate Synthase
Yonggyun Kim, Dae-Weon Lee, Jin Kyo Jung
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Abstract

A subtropical insect, Maruca vitrata (F.) (Lepidoptera: Crambidae), is invasive to temperate zones, in which low temperatures during winter would be a serious challenge for colonization. This study assessed cold tolerance and cold-hardening of M. vitrata to understand its overwintering mechanism. Supercooling capacity was confirmed in all developmental stages exhibiting body freezing points at lower than -10°C, in which supercooling points (SCPs) were significantly different among developmental stages, with eggs having the lowest SCP (at -22.5°C). However, all developmental stages suffered significant mortality after being exposed to low temperatures much higher than SCPs. Furthermore, nonfreezing injury increased with elapsed time at 25°C after cold shock. One of the nonfreezing symptoms was a darkening on thorax, which was explained by uncontrolled prophenoloxidase activation. Pre-exposure to 8°C for 1 h significantly increased the survival of both young and old larvae to a low-temperature treatment (-5°C for 1 h). Rapid cold-hardening (RCH) was accompanied by significant increase in hemolymph trehalose concentration. During RCH, trehalose-6-phosphate synthase was significantly upregulated in its expression level.These results suggest that M. vitrata is a freeze-susceptible species and becomes cold-hardy via hypertrehalosemia.

© The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Yonggyun Kim, Dae-Weon Lee, and Jin Kyo Jung "Rapid Cold-Hardening of a Subtropical Species, Maruca vitrata (Lepidoptera: Crambidae), Accompanies Hypertrehalosemia by Upregulating Trehalose-6-Phosphate Synthase," Environmental Entomology 46(6), 1432-1438, (23 September 2017). https://doi.org/10.1093/ee/nvx153
Received: 11 April 2017; Accepted: 26 August 2017; Published: 23 September 2017
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