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24 October 2017 Developmental Phase-Specific Mortality After Ultraviolet-B Radiation Exposure in the Two-Spotted Spider Mite
Yasumasa Murata, Masahiro Osakabe
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Abstract

Exposure to ambient ultraviolet-B (UVB) radiation generates DNA lesions, such as cyclobutane pyrimidine dimers and 6-4 pyrimidine–pyrimidine photoproducts in Tetranychus urticae Koch (Acari: Tetranychidae). Larvae appeared normal and healthy after UVB irradiation. Conversely, many mites were trapped in their old epidermis or experienced retarded development and shrunk, thus failing to molt from protochrysalises to protonymphs and died. This suggested that DNA lesions per se were not causing lethality in mites unless damaged genes were expressed. UVB-induced DNA lesions may have interfered with DNA replication and gene expression during the physiological changes of morphogenesis in the chrysalis stage. Comprehensive gene expression analysis by RNA sequencing revealed that gene expression involving epidermal tissue (characteristically cuticular protein genes) and myosin heavy chain muscle-like genes were downregulated in protochrysalises irradiated with UVB at the larval stage. We conclude that the success of protochrysalis molting is determined by whether the DNA lesions of genes, particularly those connected with morphogenesis, are repaired before expression at the protochrysalis stage.

© The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Yasumasa Murata and Masahiro Osakabe "Developmental Phase-Specific Mortality After Ultraviolet-B Radiation Exposure in the Two-Spotted Spider Mite," Environmental Entomology 46(6), 1448-1455, (24 October 2017). https://doi.org/10.1093/ee/nvx169
Received: 19 July 2017; Accepted: 25 September 2017; Published: 24 October 2017
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KEYWORDS
Acari
DNA lesion
protochrysalis
Tetranychidae
ultraviolet-B damage
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