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21 January 2021 Evaluation of Long-Lasting Insecticidal Nets (DuraNet®) Under laboratory and Semi-Field Conditions Using Experimental Huts Against Anopheles Mosquitoes in Jimma Zone, Southwestern Ethiopia
Brhane Gebremariam, Wondwosen Birke, Wuhib Zeine, Argaw Ambelu, Delenasaw Yewhalaw
Author Affiliations +

BACKGROUND: Long-Lasting Insecticidal Nets (LLINs) efficacy could be compromised due to a lot of influences together with user compliance and vector population insecticide resistance status. Thus, this study was to assess the biological efficacy of DuraNet® with the help of the World Health Organization cone bioassay and field experimental hut.

METHODS: A laboratory and a semi-field conditions experimental huts against Anopheles Mosquitoes were conducted in southwestern Ethiopia from September 2015 to January 2016. The bio efficacy of DuraNet® was evaluated using the WHO cone bioassay test and then its field efficacy was evaluated using experimental huts against the malaria vector population.

RESULTS: World Health Organization cone bioassay tests against pyrethroid-resistant An. arabiensis led to mean percent mortality and knockdown of 78% and 93%, respectively. Washing of DuraNet® successively reduced its efficacy from 93% knockdown (0 wash) to 45% knockdown (20 washes). Similarly, mean mortality decreased from 84% (0 wash) to 47% (20 washes). A total of 1575 female mosquitoes were collected over 40 nights out of which 1373(87.8%) were An. gambiae s.l., 116 (7.4%) were Anopheles coustani and 107 (6.8%) were An. pharoensis. The mean blood-feeding rate was significantly lower (P < .001) in hut containing unwashed DuraNet® when compared to hut containing untreated DuraNet®. The mean mortality rate was significantly higher (P < .001) in hut containing DuraNet® when compared to hut containing untreated DuraNet®. Unwashed DuraNet® showed the highest personal protection 88.7% and 100% against An. Arabiensis and An. pharoensis, respectively.

CONCLUSION: Both DuraNet® and PermaNet 2.0 moderate efficacy against a pyrethroid-resistant population of An. arabiensis from Ethiopia. The bio efficacy of DuraNet® was found below the WHO recommendation. Therefore, the real impact of the observed insecticide resistance against DuraNet® to be further studied under phase-III trials, the need for new alternative vector control tools remains critical.


Malaria is still one of highest health risks in developing countries with high morbidity and mortality in under 5 children. Assessment of overall economic impact of the disease shows that it accounts for 40% of health cost spending, 30% to 50% of inpatient charges, and up to 50% of outpatient official visits in areas with great malaria.1 According to the world health organization, there were 216 million new incidents of malaria leading to 445 000 death, of which 90% occurred in African countries.2

Long-Lasting Insecticidal Nets (LLINs) are uppermost community health apparatuses and, when used by children and pregnant women, subsidize to improving motherly, neonatal, and infant health, with long-lasting reimbursements to the emerging child.3 These nets provide personal barrier from bite by mosquitoes in addition those nets lessen also the transmission of malaria and have excito-repellency effect.4 A total of 505 million insecticidal nets (ITNs) were distributed in developing countries between 2014 and 2016 with household ownership of at least 1 insecticidal net, improved from 50% in 2010 to 80% in 2016. Nevertheless, the ratio of houses with adequate bed nets (ie, 1 net for every 2 people) is very low (43%).2 Following the high demand of bed nets as key vector control intervention, companies are ramping up production and new brand nets are being introduced for public health utilization.

In order for any new long-lasting insecticidal nets to be used in public health, it must gain WHOPES approval.5 The approval process passes through 3 stages of efficacy testing (laboratory, small-scale semi-field, and large-scale field trial). To be classified as a long-lasting insecticidal net, it must maintain their effective biological activity for at least 20 World Health Organization recommended washes typical washes under laboratory conditions and 3 years of suggested use under semi-field and field condition.

DuraNet® is an LLIN developed by the Shobikaa Impex pvt Ltd (Karur, Tamil Nadu 639006, and India). It contains 0.55% w/w ± 15% alpha-cypermethrin. The net is a polyethylene fiber coated with a proprietary polymer containing target dose of alpha-cypermethrin at 250 mg/m2. The polymer binds to the fiber and can withstand multiple washings, the active ingredient diffusing in a controlled manner to the surface of the polymer coat to maintain insecticidal efficacy.

Thus, this study was conducted to verify the intended efficacy of the product in the presence of pyrethriod resistance vector population of An. gambiae s.l. using cone bioassay and semi-field experimental huts in southwestern Ethiopia. The cone bioassays were conducted in Tropical and Infectious diseases research institute, Jimma University. The experimental hut study trials were undertaken near the Gilgel-Gibe hydroelectric power dam-I.


Study area and period

This study was conducted from September 2015 to January 2016 near the Gilgel-Gibe hydroelectric dam area, southwestern Ethiopia. The hydroelectric dam is one of the major hydroelectric dams in Ethiopia with artificial reservoir occupying an estimated area of 62 kmsq.6 It produces around 184 MWh and is located 260 km southwest of the capital Addis Ababa. It has been functional since 2004. The study area is located between latitudes 7°42′50″N and 07°53′50″N and longitudes 37°11′22″E and 37°20′36″E with an altitude ranging from 1672-1864 m above sea level. The area has a sub-humid, warm to hot climate, obtains between 1300 and 1800 mm of rainfall annually and has a mean annual temperature of 19°C. Mostly, the 2 peak seasonal transmissions of malaria occur during the months of September to December and March to May in the study area.

Bio-efficacy testing of LLIN (DuraNet®) under laboratory setting

LLIN sample preparation and washing process

DuraNet® LLINs (Karur, Tamil Nadu 639006, and India) is treated with alpha-cypermethrin at a target dose of 250 mg/m2 of netting material. It contains 0.55% W/W ± 15% alpha-cypermethrin. The alpha-cypermethrin chemical is coated onto filaments at bursting strength of 450 kPa of netting material and of 145 ± 5% for seam sub-section per denier yarn. Prior to testing the production date and batch number of all nets were recorded. Seven sub-samples per net (1 from the roof, the rest from side of the net) were taken from each net and prepared for standard LLINs cone tests by cutting 25 cm × 25 cm pieces following WHO protocol.7 In this study, 9 candidate nets were used for bio-efficacy testing. Thus, a total of 63 sub-sample net pieces (7 sub-sample pieces from each net × 9 DuraNet®s) individually rolled up in aluminum foil, labeled (by net type, net number and sample area) and kept in a refrigerator prior to the assay. Concurrently 9 (1 sub-sample piece from each untreated DuraNet® × 9 untreated DuraNet®s) sub-samples (30 cm × 30 cm) were individually rolled up in aluminum foil, labeled and kept in a refrigerator prior to the assay.

Mosquito rearing and cone bioassay testing

Anopheles mosquito larvae were collected from field sites near Gilgel-Gibe hydroelectric power production reservoir and reared to adults under standard conditions (25 ± 2oC temperature, 80 ± 4% relative humidity) in tropical and infectious diseases research institute, Sekoru campus, Jimma University, Ethiopia. For WHO cone bioassay test, five 2-5 days age unfed female An. gambiae s.l. (presumably An. arabiensis according to Yewhalaw et al., (2009))6 mosquitoes were used per cone. Twenty mosquitoes (5 mosquito’s per-cone × 4 cone-per sub-sample net piece) were introduced into the cone facing net sample for 3 minutes and then moved to holding paper cups. Then mosquitoes were supplied with a 10% sucrose solution. The number of mosquitoes knocked down and the number of dead mosquitoes were recorded every 10 minute within 60 minutes and 24 hours, respectively. Mosquitoes exposed to untreated DuraNet® pieces were used as controls and experiment conditions were set to be 27 ± 2°C temperature and 75 ± 10% relative humidity (RH) throughout the study. Thus, a total of 1260 mosquitoes (63 net sub-samples × 20 mosquitoes per sub-sample net) were used for complete bioassay testing and another 180 mosquitoes (9 untreated net sub-samples × 20 mosquitoes per sub-sample net) were used as control.

For net pieces washing each net sub-samples (25 cm × 25 cm) were introduced individually into 1-l beakers containing 0.5 l deionized water, with 2 g/l WHO recommended soap (Savon de Marseille; pH 10-11) added and fully dissolved just before washing. The beakers were then introduced into a water-bath at 30°C and shaken for 10 min at 155 movements per minute. The samples were then removed, rinsed twice for 10 min in clean, deionized water under the same shaking conditions as above, dried at room temperature and stored at 30°C in the dark between washes.

Bio-efficacy testing of DuraNet® LLIN using experimental huts

Experimental hut establishment

Five experimental huts of West African style, each with 1 room and screened veranda trap were established approximately 500 m from Gilgel-Gibe reservoir shore, southwestern Ethiopia following world health protocol developed for field evaluation of long-lasting insecticidal nets.5 The huts were made from concrete bricks with a corrugated iron roof, a ceiling of white cotton sheeting and a concrete base surrounded by a water filled moat to prevent the entry of ants. Details of the dimensions of the hut, the Verenda trap and materials from which the tukuls made were described elsewhere.8 The slits were constructed from pieces of metal shutters, fixed at an angle of 45° to create a funnel of 1 cm between slits. The design of window slit allows the inward flight of mosquitoes coming from filed but it will be hardly possible for them to escape once they entered the hut. A veranda trap made of iron mesh (22 mm diameter) was set at the back of each hut for trapping exophilic mosquitoes (Figure 1). It is presumed that some mosquitoes (inherently exophilic or mosquitoes repelled due to the exito-repellency effect of the chemicals impregnated into LLINs) will exit the hut once they enter and then after feeding on their host of choice. Thus, the verenda trap was designed to trap and quantify the proportion of mosquitoes exit the experimental hut and by virtue the efficacy of LLINs under test for its exito-repellent effect. Each night mosquitoes were allowed to enter into the hut via the window slits from the environment and freely move between the room and the verenda trap.

Figure 1.

Experimental hut design of the study.


Treatment arms and sleepers rotation

In this experiment, 5 different treatment arms were used. These include (1) Untreated unwashed DuraNet® (Negative control) (2) Unwashed treated DuraNet® (3) treated DuraNet® 20 times washed (4) PermaNet® 2.0 20 times washed, and (5) unwashed PermaNet® 2.0 (positive control). All nets were 75 denier polyethylene and polyester nets respectively. To simulate wear and tear condition of nets under usage 6 (4 cm × 4 cm size) holes were cut in each net (2 holes on each of the sides and 1 hole at each end). The DuraNet® LLIN and PermaNet® 2.0 were washed according to World Health Organization Stage II washing procedure.5 For washing, the nets were put in to aluminum bowl with 10 l of tap water and 2 g/l of savon de Marseille soap. The nets were agitated for 3 minutes, then soaked for 4 minutes, and agitated again for 3 minutes. The nets were agitated manually by stirring them with a pole at 20 rotations per minute. Thus, each net was washed for a total of 10 minute and then rinsed with clean water by a similar procedure, dried horizontally in the shade and stored at ambient temperature between washes. WHO recognized PermaNet® 2.0 LLIN washed 20 times, was used as a positive control to assess DuraNet® LLIN performance.

In each data collection night, 2 non-smoker male volunteers aged 20 to 25 were allowed to sleep in each experimental hut with its door remain closed between 19:00 and 07:00 hours. Each team was rotated between treatments on successive nights within a week to avoid possible bias which could arise due to individual attractiveness to mosquitoes. There were 5 successive collection nights (Monday to Friday) and 2 successive break nights for hut ventilation per week. Thus, there were 25 collection nights in order to complete the whole study. Informed written consent was obtained from each sleeper.

Mosquito collection, identification and key parameters measured in determination of LLINs efficacy

Mosquitoes were collected from 6:00 to 7:00 each morning inside bed nets, floors, walls, ceilings, and verandas of each experimental hut by the help of mouth aspirators and torches. Then the collected mosquitoes were recorded as dead or alive. Live mosquitoes were held in paper cups and supplied with 10% sucrose solution. The collected mosquitoes were transported to Asendabo Vector Biology Laboratory, Jimma University, where mosquitoes were sorted by genus, sex and morphologically identified using taxonomic keys.9 Mosquitoes were also scored for their physiological state as unfed, fed, half gravid and gravid. Delayed mortality was recorded after 24 hours. To evaluate the efficacy of DuraNet LLIN against the resistant populations of An. arabiensis, different entomological parameters (deterrence, exit, blood feeding inhibition, and mortality rates) were derived from basic measurements.

The primary outcomes were:

  • 1. Deterrence—the reduction in entry in to treatment hut relative to the control hut (ie, huts holding untreated nets);

  • 2. Mortality—the proportion of mosquitoes killed relative to the total catch size;

  • 3. Killing effect—the numbers killed by a treatment relative to the untreated control, as derived from the formula;


Where Kt is the number dead mosquitoes in the huts with treated nets, Ku is the number dead mosquitoes in the huts with untreated nets, and Tu is the total entering the huts with untreated nets.

  • 4. Blood Feeding Inhibition–The proportional reduction in blood feeding in huts with treated nets relative to controls with untreated nets.

  • 5. Personal Protection—The reduction in mosquito biting by treated nets relative to untreated nets, as derived from the formula


Where Bu is the total number blood fed mosquitoes in the huts with untreated nets, and Bt is the total number blood fed in the huts with treated nets.

Data analysis

Association between response variables (Percent knockdown and percent mortality) and wash time was analyzed using linear regression. We compared the number of mosquitoes entered to the different treatment arms using negative binomial regression model. Proportion of mosquitoes entering in to the treatments like Mortality, blood feeding, and exiting were estimated by using logistic regression model.


Cone bioassay and washing resistance

Exposure of wild population of An. arabiensis mosquitoes to net sections of DuraNet® LLIN in WHO bioassay tests gives mean mortality rate of 78% as well as mean knockdown effect of 93%. Mean percent knockdown and percent mortality of DuraNet® LLIN showed significant relationship (R2 = 0.797, n = 260, P < .001). The wash resistance activity of DuraNet® LLINs, measured in terms of percent knockdown and mortality is presented in Figure 2. The mean percent knockdown of wild population of An. arabiensis decreased from 93% to 45% up on exposure to the DuraNet® washed 0 and 20 times respectively. The mean percent mortality decreased from 84% to 47% on exposure to the DuraNet® washed 0 and 20 times, respectively. Wash resistance has negative correlation with percent knockdown and percent mortality which means as number of wash increase, percent knockdown and percent mortality declined (r2 = 0.333, %KD = 89.16 ± 2.15, P < .001 and r2 = 0.236, %Mt = 81.94 ± 1.68, P < .001), respectively.

Figure 2.

Mean percent knockdown and mortality of An. arabiensis exposed in 3 minutes cone bioassay to DuraNet®, Jimma, and southwestern Ethiopia.


Experimental hut trial

Mosquito entry into the huts

A total of 1575 Anopheles mosquitoes were entered and collected in the 40 nights during the trial. These consisted of 1373 (87.8%) An. gambiae s.l. presumably An. arabiensis (Yewhalaw et al., 2009), 116 (7.4%) Anopheles coustani (Laveran), and 107 (6.8%) An. pharoensis (Theobald). The mean number of mosquitoes caught per night was 8.75 for An. arabiensis, 5.52 for An. Coustani, and 6.33 for An. pharoensis. The mean mosquito entry is not significantly different among the treatment arms (F = 1.277, P > .05). There was no clear evidence of deterrence associated with any of the treatments however; there were fewer An. Arabiensis in huts with the Unwashed DuraNet® compared to treated DuraNet® and PermaNet® 2.0 (Table 2).

Mosquito mortality, blood feeding and exit rates

Mosquito blood feeding rates, mortality rates and exit rates of the 5 treatments are presented in Table 1. The mean blood feeding rate was significantly lower (P <.001) in hut containing unwashed DuraNet® when compared to hut containing untreated DuraNet®. However, there was no significance difference (P >.05) in blood feeding rate among huts containing untreated DuraNet®, 20 times washed DuraNet®, unwashed PermaNet® 2.0 and 20 times washed PermaNet®2.0. The mean mortality rate was significantly higher (P < .001) among huts containing treated DuraNet®, 20 times washed DuraNet®, unwashed PermaNet® 2.0 and 20 times washed PermaNet® 2.0 when compared to hut containing untreated DuraNet®. Higher mean number of mosquitoes were caught while exiting the huts containing treated DuraNet®, 20 times washed DuraNet®, unwashed PermaNet® 2.0 and 20 times washed PermaNet® 2.0 when compared to hut containing untreated DuraNet®, however, there was no significant difference (P > .05) among the treatments.

Table 1.

Mean blood feeding, mortality and exit rates of populations of An. arabiensis exposed to different bed net types in field experimental huts in southwestern Ethiopia.


Blood feeding inhibition and personal protection

Unwashed DuraNet® showed strong and better bio-efficacy in terms of blood feeding inhibition when compared to untreated DuraNet® and the rest of the treatment arms. The mean blood feeding rate of mosquitoes collected from a hut with unwashed DuraNet®, Unwashed PermaNet 2.0, twenty times washed DuraNet®, 20 times washed PermaNet 2.0 and Untreated DuraNet®, was 0.8/person-night, 4.1/person-night, 5.7/person-night, 6.0/person-night, and 7.0/person-night respectively. There was significant reduction (P < .001) in blood feeding inhibition in all the treatment arms (hut with unwashed DuraNet®, hut with 20 times washed DuraNet®, hut with unwashed PermaNet 2.0, hut with 20 times washed permanent 2.0) when compared to hut with untreated DuraNet®.(control). Blood feeding inhibition can be also expressed as personal protection and unwashed DuraNet® showed the highest personal protection 88.7%, 100%, and 93.3% against An. arabiensis, An. pharoensis, and An. coustani, respectively. Both PermaNet 2.0 twenty times washed and DuraNet® washed twenty times showed low to moderate personal protection (45.6% against An. arabiensis to 83% against An. pharoensis) when compared to untreated DuraNet® (Table 2).

Table 2.

Blood-feeding inhibition and personal protection due to DuraNet® LLIN in the experimental huts, southwestern Ethiopia.


The killing effect DuraNet®

DuraNet® (both unwashed and 20 times washed) showed low to moderate killing efficacy (65.93% to 54.03%) against vector population of An. arabiensis. Whereas, against An. pharoensis, both unwashed and washed DuraNet® showed no evidence of killing effect (0.00 to 9.0%). Similarly, PermaNet 2.0 (unwashed and washed) showed low to moderate killing effect against population of An. arabiensis (Table 3).

Table 3.

The overall killing effect of DuraNet® LLIN in experimental hut.



Insecticide resistance remains major threat against the efficacy of long-lasting insecticidal nets, which are key intervention tools in controlling malaria vector. In this study laboratory bioassay was initially conducted using field collected larvae raised to adult population of An. arabiensis to assess the bio-efficacy of unwashed DuraNet® (candidate bed net) and untreated DuraNet® (negative control). Furthermore, field experimental study was conducted on bio-efficacy of unwashed DuraNet® and PermaNet 2.0 following WHOPES standard guidelines in order to corroborate the laboratory findings.5

The cone bioassay tests indicated that exposure of population of An. arabiensis mosquitoes from Gilgel-Gibe area, southwestern Ethiopia, to sections of DuraNet® LLIN resulted in mean knockdown and mean mortality of 93% and 78%, respectively. The mortality result is slightly lower than the range of WHO recommendation (>80%) for public health application. The knockdown effect also is slightly below the required WHOPES recommended levels of 95% and above. In contrast to the above results earlier bio efficacy tests conducted using unwashed DuraNet® in India (Sood et al10; Gunasekaran et al11) showed 100% efficacy when tested against populations of different species of anopheles. The slight decline in bio-efficacy of DuraNet® in this study may be due to resistance development of vector populations of An. arabiensis in the study area.8,12 In Ethiopia, Previous studies indicate that populations of An. Arabiensis have developed resistance against insecticides used for net impregnation.1317

Wash resistant long-lasting insecticidal nets treated with pyrethroids are viewed as an important device in the area of vector control that would lessen the difficulties related to re-treating conventional insecticide treated nets.18 DuraNet®, the alpha-cypermethrin treated net, has similar conditions as that of Interceptor® LLIN and PermaNet® 3.0, which had been given interim but full recommendation since June 2017 by WHOPES.19

In current study, DuraNet® showed reduced washing resistance. This is reflected in continues decrease of both mean percent knockdown and mean percent mortality of population of An. arabiensis on exposure to the DuraNet® washed 0 and 20 times, respectively. This is in contrast to the study done by Sood et al10 which showed no significant difference in percent mortality and percent knockdown of population of An. culicifacies between unwashed and 20 times washed DuraNet® in India. However, in the same study by Sood et al,10 DuraNet® showed 100% knockdown but only 45% mortality after 20 washes against Anopheles gambiae which is also consistent with current results. Wash resistance technology is added to the LLIN’s materials with the assumption of pyrethroid chemicals such as Alpha-cypermethrin should have a strong affinity to the polyester netting fibers so that even after forceful washing a thin layer of pyrethroid, practically undetectable by High Performance Liquid Chromatography yet adequately bio active to encourage knockdown and mortality should still continue bound to the fibers.

Comparison of mosquito density collected from experimental huts with (unwashed DuraNet®, 20 times washed DuraNet®, unwashed PermaNet®2.0, 20 times washed PermaNet®2.0, and untreated DuraNet®) showed no clear evidence of deterrence associated with any of the treatments however; there were fewer An. Arabiensis in huts with the unwashed DuraNet® compared to untreated DuraNet®. Likewise, a study carried out by Asale et al8 using experimental huts in the study area showed the absence of significance variation in deterrence among huts containing unwashed PermaNet®2.0, untreated net and DDT sprayed hut. The possible explanation for the absence of significant variation in mosquito density among treatment arms could be accounted to low number of mosquito catches during the study period but the impact of insecticide resistance on the efficacy of the nets to be further studied in phase-III trials.

In this study significant reduction in blood feeding rate was observed in hut containing unwashed DuraNet® when compared to hut containing untreated DuraNet®. Similarly, higher mean mosquito mortality rate was recorded among huts containing treatment arms when compared to hut containing control net (untreated DuraNet®). The results for DuraNet® are new report from Jimma area thus we could not corroborate but, Vector population of An. arabiensis from the study area showed no significant reduction of mortality rate, exit pattern and blood feeding inhibition when tested against PermaNet 2.0.8

In this study, DuraNet® and PermaNet 2.0 showed low to moderate killing efficacy against vector population of An. arabiensis. Moreover, both DuraNet® and PermaNet 2.0 showed no evidence of killing effect against An. pharoensis. According to Kitau et al20 LLINs and ITNs treated with pyrethroids were more effective at killing An. gambiae and An. funestus than An. arabiensis. Thus, the variations in the outcome variables of Anopheles shown above (DuraNet® and PermaNet 2.0) may be due to different susceptibility status and species differences.


In this study, supplementary test like chemical assays were not conducted to measure the total insecticide content of the netting before and after wash resistance studies that support better interpretation of the results.


Cone bioassay study of DuraNet® LLINs against population of An. arabiensis in Jimma area showed reduced bio-efficacy when measured in terms of percent knockdown rates which were slightly below the World Health Organization recommendation. The evaluation of the efficacy of DuraNet® and PermaNet 2.0 LLINs using experimental huts showed that both vector control tools showed low to moderate efficacy against pyrethroid resistant population of An. arabiensis from Ethiopia. While the real impact of the observed insecticide resistance against key vector control tool (LLINs) to be further studied under phase-III trials, the need for new alternative vector control tools remains a matter of urgency.


We are thankful to the participants who slept in the experimental huts and Jimma University for financial support.

Author’s contributions

BG, AA, and DY conceived, designed and participated in conducting the study. WB, WZ, and DY were involved in data management, analysis, interpretation, and drafting the document. All authors read and confirmed the final document.

Ethical considerations

The study proposal was reviewed and approved by the institutional review board (IRB) of the College of Health Sciences, Jimma University (Reference Number RPGC/344/06).



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© The Author(s) 2021 This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (
Brhane Gebremariam, Wondwosen Birke, Wuhib Zeine, Argaw Ambelu, and Delenasaw Yewhalaw "Evaluation of Long-Lasting Insecticidal Nets (DuraNet®) Under laboratory and Semi-Field Conditions Using Experimental Huts Against Anopheles Mosquitoes in Jimma Zone, Southwestern Ethiopia," Environmental Health Insights 15(1), (21 January 2021).
Received: 16 August 2020; Accepted: 13 October 2020; Published: 21 January 2021

Anopheles arabiensis
Anopheles gambiae
cone bioassay
experimental hut
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