Open Access
How to translate text using browser tools
1 June 2008 Effects of the Exotic Crustacean, Armadillidium vulgare (Isopoda), and Other Macrofauna on Organic Matter Dynamics in Soil Microcosms in a Hardwood Forest in Central Florida
Jan Frouz, Richard Lobinske, Jirí Kalcík, Arshad Ali
Author Affiliations +

Soil biota play an important role in the transformation of soil organic matter (SOM), affecting its distribution in the soil profile, plus directly or indirectly affecting a large set of other soil parameters, such as water holding capacity and nutrient availability (Lavelle et al. 1997; Ponge 2003). Some microflora play a primary role in the decomposition and chemical transformation of SOM, whereas soil macrofauna contribute little to the mineralization of SOM, but can significantly affect distribution of SOM in the soil profile by litter fragmentation and its mixing with mineral soil, and thus indirectly affecting soil microflora (Anderson & Ineson 1984; Lavelle et al. 1997). The effects of soil macrofauna on organic matter removal from the litter layer have been the subject of many studies (e.g., Irmer 1995; Carcamo et al. 2001). However, relatively less attention has been paid to the quantification of the effects of soil fauna on organic matter stabilization and accumulation in mineral soil (Wolters 2000). Species replacement or displacement during succession may cause substantial changes in the transformation of SOM and formation of the upper soil layer (Dunger 1991; Rusek 1978; Frouz et al. 2001). Invasion by exotic species may produce changes, as exemplified by the extensive studies of Scheu & Parkinson (1994) and Boehlen et al. (2004) of an earthworm introduced into north temperate forests. However, fewer data are available on the ecological significance of invasion by other exotic soil invertebrates. Armadillidium vulgare (Latreille) is a common European terrestrial isopod that has been introduced into many locations in the USA (Ellis et al. 2000; Stoyenoff 2001), with reports of prevailing densities as high as 10,000 individuals per m2 (Frouz et al. 2004). Terrestrial isopods are important macrosaprophagous organisms that may consume significant amounts of litter (Zimmer 2002).

The objective of this microcosm study was to elucidate the effects of soil macrofauna on SOM decomposition and accumulation in the mineral layer in a central Florida hardwood forest with specific reference to the role of A. vulgare in these processes. Field-placed microcosms facilitated the use of different treatments and the replication of treatments and controls.

The study was conducted in a hydric hardwood forest on sandy soil along the shore of Lake Apopka (28°38’18.54”N, 81°33’04.50”W), near the Mid-Florida Research and Education Center of the University of Florida at Apopka, Florida. In this area, Celtis laevigata Willd. was the major overstory tree with a scattering (∼10%) of Carya glabra (Mill.). The major understory trees were Prunus caroliniana Aiton (70%), and small Celtis laevigata and Sabal palmetto (Walter) Lodd. ex Schult. & Schult. f. (20%).

The microcosms used in this study were similar in construction to those used previously by Frouz et al. (2006). Each microcosm consisted of a clear plastic box (18 × 25 × 5 cm) with twelve nylon net (0.3 mm) covered openings (1-cm diameter) on the top and bottom surfaces. Each box contained 100 g dry weight (DW) mineral sand and 10 g DW autochthonous litter in a 2-mm mesh litter bag with eight 1-cm diameter openings. Litter was collected from the study site forest floor at the planned exposure location of each microcosm, hand sorted, cut into about 1- × 3-cm pieces and mixed. Sand was collected from deeper soil layers (20-30 cm deep) at the same locations, sieved to remove roots, and washed several times in water. Both litter and sand were air dried before use. Separate samples were taken from each microcosm to determine dry weight and carbon content. Three treatments with 4 replicates each of different access for soil macrofauna to the microcosms were established. In the macrofauna accessible (MA) units, 6 horizontal openings (4 × 30 mm) were located in each box along lateral side. No openings were made in the macrofauna non-accessible (MN) boxes, or in the A. vulgare-colonized (AC) boxes to which 5 mature specimens of A. vulgare were added. This represented a density of ca.100 individuals per m2, which was the mean density of A. vulgare in forest litter layer at the place and time of exposure of experimental boxes. This density was established by taking five 18- × 25- cm area samples of litter, and hand sorting the samples for A. vulgare which were introduced in equal numbers into the individual boxes. Boxes were exposed in the field for 3 months, from Jul 15 to Oct 15, 2006. This period covers warmest and wettest months of the year where decomposition is likely to be the fastest. After exposure, the litter bags were removed from the boxes and the litter bag contents, as well as the mineral layer, were air dried and weighed. From both layers of each box, 2 samples were taken for measurement of dry weight and carbon (C) content. Dry weight was determined gravimetrically; litter was dried to constant dry mass at 90°C and mineral samples at 105°C. The lower temperature was used for litter drying to avoid weight loss by possible volatilization of aromatic compound. Carbon content was established as oxidizable carbon content (Cox), determined by the wet acidified dichromate oxidation method of Jackson (1958). Carbon stock in an individual soil layer was calculated as the layer DW multiplied by the corresponding C concentration. Carbon removal from litter bag contents was calculated as the difference between C stock in the litter bag at the beginning and at the end of the experiment. Incorporation of C into the mineral layer was calculated as the difference between the mineral layer C stock at the end of experiment and the start of experiment. Total loss of carbon from the box was calculated as difference between C stock in both layers at the start and end of exposure period.

Available P, K, and NO3- ions as well as soil pH were measured in the mineral layer at the end of exposure period. Soil pH was measured in KCl solution (ISO 1992); K was measured by ion selective electrodes (type 20-19) in citric acid solution, NO3- by ion selective electrodes (type 20-31) in KCl (Frant 1994); in all cases 1:5 sample solvent ratio was used. The electrodes were manufactured by Electrochemical Detectors, Turnov, Czech Republic. Available P was detected by ion exchange resin, which is assumed to approximate P available for plants (Machacek 1986).

Values of dry mass as well as C stock (mass × content) in litter bag contents had decreased significantly at the end of the experiment compared to the initial values, indicating significant losses of C from litter bag contents in all treatments (Table 1, Fig. 1). In agreement with other litter bag studies which indicate that the presence of macrofauna increases litter decomposition rates by 5-40% (Irmer 1995), macrofaunal accessibility to the microcosms in this study significantly increased dry mass and C removal from litter bag contents (Table 1).

Dry mass of the mineral layer did not differ significantly among treatments, although in MN-treated microcosms, the mean mass of the mineral layer was lower than the other treatments. We suspect that this was caused by rain, washing small particles from the mineral layer as happened in all treatments. However, in the MA and AC microcosms, this loss of mineral content was compensated by the incorporation of organic matter from the litter bag. This idea is supported by the significantly higher C content in MA treatment than in the MN treatment (Table 1). In addition, incorporation of C in the mineral layer was significantly higher in the MA treatment than in the other 2 treatments (Fig. 1). The MA-treated microcosms tended towards the lowest overall system loss of C, although the differences were not statistically significant (Fig. 1). In agreement with previous experiments with similar enclosures (Frouz 2002; Frouz et al. 2006), we can conclude that access by soil macrofauna increased removal of C from the litter layer but not the overall mineralization, as most of the C which is removed from litter was stored in the mineral layer. Similar conclusions were made by Wachendorf et al. (1997) after comparing total mass loss with the mass loss caused by microbial and animal respiration in litter bags. Soils in the MA treatment also had significantly higher pH and content of available P and K than the MN treatment (Table 2). This is in agreement with other studies indicating that incorporation of organic matter into mineral soil by macrofauna in the MA treatment may substantially alter other soil properties, such as pH and water holding capacity (Frouz et al. 2006).

By comparing microcosms occupied by A. vulgare with those accessible to all macrofauna, we can see that A. vulgare plays an important role in the overall macrofaunal effect. This is more pronounced in C removal from the litter than in C incorporation into the mineral layer. Armadillidium vulgare activity had a strong effect on the chemistry of the mineral layer as indicated by increased pH level and content of available P, K, and NO3- content. This suggests a significant role of A. vulgare in the transformation of the topsoil layer, and this effect may be more pronounced at higher densities of A. vulgare. In some other locations in Florida, densities of A. vulgare were several orders of magnitude higher (up to 10,000 individuals per m2) (Frouz et al. 2004), than in the present study. Thus, in some cases, A. vulgare invasion may significantly alter soil conditions and this poses a new question: How much does the effect(s) of A. vulgare activity influence the performance of other plant or animal species co-existing with this isopod?

Gratitude is expressed to Dr. Richard Beeson, Mid-Florida Research and Education Center, University of Florida, for help in taxonomic determination of trees at the study site. The study was partly supported by the Czech Republic research plan of ISB BS AS CR No Z6066911, and a Fulbright grant to the senior author at MREC, University of Florida.


In a study of the effects of the non-native Isopod Armadillidium vulgare on soil organic matter decomposition and incorporation into the mineral soil of field microcosm in a central Florida hydric hardwood forest, that species was found to have an important effect on the transformation of the topsoil layer. This was evidenced by increased pH, P, K and NO3- in the mineral layer and increased removal of C from leaf litter layer.

References Cited


J. M. Anderson and P. Ineson . 1984. Interaction between microorganisms and soil invertebrates in nutrient flux pathways of forest ecosystems. pp. 59-88 In J. M. Anderson, A. D. Rayner, and D. W. H. Walton [eds.], Invertebrate Microbial Interactions. Cambridge University Press, Cambridge. Google Scholar


P. J. Boehlen, P. M. Groffman, T. J. Fahey, M. C. Fisk, E. Suarez, D. M. Pelletier, and R. T. Fahey . 2004. Ecosystem consequences of exotic earthworm invasion of north temperate forests. Ecosystems 7:1–12. Google Scholar


H. A. Carcamo, C. E. Prescott, C. P. Chanway, and T. A. Abe . 2001. Do soil fauna increase rates of litter breakdown and nitrogen release in forests of British Columbia, Canada? Canadian J. For. Res 31:1195–1204. Google Scholar


W. Dunger 1991. Zur Primärsukzession humiphager Tiergruppen auf Bergbauflächen. Zool. Jahrb. Syst 118:423–447. Google Scholar


L. M. Ellis, M. C. Molles, C. S. Crawford, and F. Heinzelmann . 2000. Surface-active arthropod communities in native and exotic riparian vegetation in the middle Rio Grande Valley, New Mexico. Southwest Naturalist 45:456–471. Google Scholar


FrantM. S 1994. History of the Early Commercialization of Ion-Selective Electrodes. Analyst 199:2293–2301. Google Scholar


J. Frouz, B. Keplin, V. Pizl, K. Tajovsky, J. Stary, A. Lukesová, A. Nováková, V. Balík, L. Hánel, J. Matena, C. Düker, J. Chalupsky, J. Rusek, and T. Heinkele . 2001. Soil biota and upper soil layers development in two contrasting post-mining chronosequences. Ecol. Eng 17:275–284. Google Scholar


J. Frouz 2002. The effect of soil macrofauna on litter decomposition and soil organic matter accumulation during soil formation in spoil heaps after brown coal mining: A preliminary result. Ekologia 21:363–369. Google Scholar


J. Frouz, A. Ali, J. Frouzova, and R. J. Lobinske . 2004. Horizontal and vertical distribution of soil macroarthropods on spatio-temporal moisture gradient in subtropical central Florida. Environ. Entomol 33:1282–1295. Google Scholar


J. Frouz, D. Elhottová, V. Kuráz, and M. Sourková . 2006. Effect of soil macrofauna on other soil biota and soil formation in reclaimed and unreclaimed post mining sites: Results of a field microcosm experiment. Appl. Soil Ecol 33:308–320. Google Scholar


U. Irmer 1995. Die Stellung der Bodenfauna im Stoffhaushat schleswig-holstein Walder. Faunistische und Oekeologische Mitteilungen, Supplement 18:1–184. Google Scholar


ISO 1992. Soil Quality, Determination of pH. ISO/DIS 10390, International Organization for Standardization. Google Scholar


M. L. Jackson 1958. Soil Chemical Analysis. Prentice Hall, Inc., Engelwood Cliffs, New York. Google Scholar


P. Lavelle, D. Bignell, M. Lepage, V. Wolters, P. Roger, P. Ineson, O. W. Heal, and S. Dhillion . 1997. Soil function in changing world: the role of invertebrate ecosystem engineers. European J. Soil Biol 33:159–193. Google Scholar


V. Machácek 1986. Verification of new method determining the rate of phosphorus release from soil. Rostlinná Vyroba 32:473–480. Google Scholar


J. F. Ponge 2003. Humus form in terrestrial ecosystem: a framework to biodiversity. Soil Biol. Biochem 35:935–945. Google Scholar


J. Rusek 1978. Pedozootische Sukzessionen wärend der Entwicklung von Ökosystemen. Pedobiologia 18:426–433. Google Scholar


S. Scheu and D. Parkinson . 1994. Effect of invasion of aspen forest by Dendrobaena octaedra (Lumbricidae) on plant growth. Ecology 75:2348–2361. Google Scholar


J. L. Stoyenoff 2001. Distribution of terrestrial isopods (Crustacea: Isopoda) throughout Michigan: Early results. Great Lakes Entomol 34:29–50. Google Scholar


C. Wachendorf, U. Irmler, and H. P. Blume . 1997. Relationships between litter fauna and chemical changes of litter during decomposition under different moisture conditions. pp. 135-144 In G. Cadisch and K. E Giller [eds.], Driven by Nature: Plant Litter Quality and Decomposition. Walingford. Google Scholar


V. Wolters 2000. Invertebrate control of soil organic matter stability. Biol. Fertility Soils 31:1–19. Google Scholar


M. Zimmer 2002. Nutrition in terrestrial isopods (Isopoda: Oniscidea): an evolutionary-ecological approach. Biol. Rev 77:455–493. Google Scholar


Fig. 1.

Mean ± SD values of carbon loss from litter layer, carbon incorporated into mineral layer, and carbon total loss from experimental microcosms after 3 months exposure (Jul 15 to Oct 15, 2006) in a central Florida hardwood forest. Microcosms were either accessible to forest macrofauna, non-accessible to forest macrofauna, or non-accessible to forest macrofauna and colonized with Armadillidium vulgare. Values of same parameter labeled by different letter are statistically different (ANOVA, LSD test, P < 0.05.).


Table 1.

Mean ± SD values* of carbon content and dry mass in leaf litter and mineral soil layers of experimental microcosms (macrofauna non-accessible; macrofauna accessible; and Armadillidium vulgare-colonized) at the beginning (initial) and at the end (final) of 3 months exposure (Jul 15 to Oct 15, 2006) in a central Florida hardwood forest.


Table 2.

Mean ± SD values* of ph (KCL), available P, K, and NO3- in the mineral layer of experimental microcosms at the end of 3 months exposure (Jul 15 to Oct 15, 2006) in a central Florida hardwood forest.

Jan Frouz, Richard Lobinske, Jirí Kalcík, and Arshad Ali "Effects of the Exotic Crustacean, Armadillidium vulgare (Isopoda), and Other Macrofauna on Organic Matter Dynamics in Soil Microcosms in a Hardwood Forest in Central Florida," Florida Entomologist 91(2), 328-331, (1 June 2008).[328:EOTECA]2.0.CO;2
Published: 1 June 2008
Back to Top