The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an economically important pest of citrus in the United States, Asia and other parts of the world, as a vector of huanglongbing (HLB) or citrus greening, which is considered one of the world's most serious diseases of citrus (Gottwald 2010). Additionally, large populations of ACP can damage plants directly through feeding and excretion activities; ACP feeds on citrus phloem tissues and produces copious amounts of honeydew excretions (Brlansky & Rogers 2007). ACP adults can feed on mature citrus leaves, but nymphs must have young tender flush to survive. For biological and pathogen-vector relation studies on ACP (e.g., Wenninger & Hall 2007; Pelz-Stelinski et al. 2010) rearing of single or small groups of psyllid nymphs or adults on whole citrus plants takes considerable space, time and other resources. Here, we describe a new, simpler method for short-term rearing of ACP using detached mature citrus leaves for adults and detached young terminal shoots for nymphs (Fig. 1).
ACP adults and nymphs were reared singly or in small groups (5–10 per tube) in clear plastic (polypropylene) 50-ml conical centrifuge tubes (3 cm wide and 11.5 cm long; Fisher Scientific, Pittsburg, PA). Young psyllid adults (approximately 1wk-old) were reared on detached, mature, medium-size leaves of sweet orange (Citrus sinensis (L.) Osbeck var. Ridge Pineapple). Leaf sizes used for adults ranged between 3–4 cm in width and 6–8 cm in length with petioles about 2–3 cm long. The petioles of these leaves were cut diagonally with a sharp razor blade, and each inserted in a small (0.3 or 0.5 mL) microfuge tube filled with water or a piece of moistened cotton wool (Figs. 1A and 1E). A piece of Parafilm membrane was wrapped around the top of this tube and the petiole to keep insects from drowning or contact with water. The detached leaf and microfuge tube were then inserted into the rearing tube, and the psyllid adults were added to the latter. The rearing tube was covered with a screw cap that had been finely perforated by a hot needle for ventilation. For better ventilation, however, wider holes can be cut in the plastic cap and a piece of fine mesh screen placed under the screw cap to prevent escape of the psyllids. The tubes were kept in an environmental chamber at 25°C and 14 h light per day.
The clear plastic wall of the rearing tubes allowed close observation and photography of the enclosed psyllids during their various activities either by the naked eye or through a stereomicroscope with minimal disturbance (Figs. 1B–1E). For example, adults were observed feeding for long periods in their normal feeding posture (Fig. 1B) mainly on the midrib or other veins on either side of the detached leaves. They were also observed excreting honeydew droplets regularly (Fig. 1E), and occasionally laying eggs (Fig. 1B inset), although they normally prefer younger leaves for laying eggs (Brlansky & Rogers 2007). Survival of young adults under the above conditions on detached mature leaves, that were changed to fresh ones weekly, was 89, 80 and 75% after 2, 3, and 4 weeks, respectively (n = 130 adults). It was later observed, however, that mature leaves can stay fresh in the rearing tubes at least for 2 weeks.
Young ACP nymphs were reared on younger citrus leaves using the above described rearing tubes under similar conditions. We followed the survival and adult emergence of young (2nd-3rd-instar) nymphs for 1 week on the following 3 types (leaf age/size) of sweet orange leaves (Fig. 1A): (A) partially expanded young leaves (2–3 terminal leaves on a young flush shoot); (B) fully expanded tender leaves; and (C) mature mid-size leaves similar to those used for rearing adults. After gently placing a group of young nymphs on each of these three types of leaves (10 nymphs/tube), the tubes were kept horizontally for a few h to overnight to allow the young nymphs to settle and start feeding. The overall proportion of nymphs that survived for 1 week in these tubes was 77.8% in treatment A (youngest leaves), 61.1% in treatment B (young leaves), and 56.7% in treatment C (mature leaves). Chi square (χ2) analysis indicated that survival of nymphs was significantly higher in treatment A than in treatments B or C (Table 1). The proportion of nymphs that turned into adults during 1 week of rearing was significantly higher in Treatments A and B (55.7–67.3%) compared to that in treatment C (25.5%) (Table 1). Survival and development of nymphs in treatment A are comparable to those of ACP nymphs reared on whole citrus seedlings at 25°C (Liu & Tsai 2000). Nymphs were observed feeding regularly for long periods, molting and excreting large amounts of honeydew in droplet or tubular forms especially on the youngest or younger leaves tested (Figs. 1C and 1D).
When the rearing tubes were kept vertical, most of the ACP honeydew excretion droplets fell down from the leaves and accumulated in the conical bottom of the rearing tubes (Fig. 1E). This can be a convenient and efficient way to collect the psyllid excretions for various studies on feeding behavior/chemistry (Hall et al. 2010). We believe that this new rearing method allows closer observation of psyllids, and can save time, space and other resources in various studies on the biology and management of ACP and probably other citrus psyllids. It can be particularly valuable for studying psyllid behavior, HLB pathogen-vector interactions, and for bioassay of biological or chemical agents against citrus psyllids.
SURVIVAL AND ADULT EMERGENCE OF YOUNG (2ND-3RD-INSTAR) NYMPHS OF D. CITRI FOR 1 WEEK IN REARING TUBES WITH DETACHED SWEET ORANGE LEAVES OF VARIOUS AGES.
We thank Kathy Moulton and Monty Watson for technical assistance. This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. Funds for this research were provided by the Florida Citrus Research and Development Foundation.
We developed a new simple method for shortterm rearing of the Asian citrus psyllid (ACP) using detached citrus leaves in 50-mL conical polypropylene tubes. Survival of young adults was 89, 80, and 75% after 2, 3, and 4 weeks, respectively, on detached mature leaves that were changed weekly. Survival and adult emergence of 2nd to 3rd-instar nymphs were significantly higher when reared on younger leaves compared to those reared on mature leaves. Honeydew excretion droplets of ACP accumulated and may be easily collected from the conical bottom of the rearing tubes. This new method allows closer observation and photography of psyllid nymphs and adults with minimal disturbance, and it can save time, space and other resources in various studies on the biology, behavior, management and pathogen-vector interactions of ACP and probably other citrus psyllids.
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