The red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae), is an invasive ant of serious public health significance. In this study, a device and a protocol were developed for collecting brood of the red imported fire ant in the field. Field trials showed that within 30 min about 700 larvae and pupae plus about one half this number of workers were collected per device. This collection procedure takes advantage of the innate brood rescue behavior of alarmed workers, which involves strong negative phototaxis. This brood collection device is not useful for collecting queens, but it provides a practical means to separate the brood from the mound soil.