We recorded 2 new collection localities of the nonnative centipede Rhysida longipes longipes (Scolopendromorpha: Scolopendridae: Otostigminae) and documented for the first time that a breeding population is established in South Florida, USA. Specimens of several size classes were collected in a large property on Islamorada, Upper Matecumbe Key, Florida, USA. Further work is necessary to assess the extent of its range and its impact on the local ecosystem.