Previously, we showed that dexamethasone (DEX) inhibited the expression of inflammatory cytokines, matrix metalloproteinase-1, and cyclooxygenase-2 messenger ribonucleic acid in SW982 cells. In this study, the effect of DEX on the transcription factors nuclear factor–κB (NF-κB) and activator protein–1 (AP-1) was examined in SW982 cells by electrophoretic mobility shift assay (EMSA). Both NF-κB and AP-1 deoxyribonucleic acid binding activities were detectable in SW982 cells by EMSA, and they were induced by interleukin-1β treatment. DEX inhibited NF-κB binding activity at 10 μM as well as at 100 μM, although the inhibition was only partial. However, DEX had little effect on AP-1 activity. These results suggest that DEX reduces the expression of inflammatory cytokines and other proteins in SW982 cells by inhibiting NF-κB.
How to translate text using browser tools
1 March 2005
EFFECT OF DEXAMETHASONE ON BINDING ACTIVITY OF TRANSCRIPTION FACTORS NUCLEAR FACTOR-κB AND ACTIVATOR PROTEIN-1 IN SW982 HUMAN SYNOVIAL SARCOMA CELLS
TAISUKE YAMAZAKI,
TAKASHI TUKIYAMA,
TAKAYOSHI TOKIWA
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular & Developmental Biology - Animal
Vol. 41 • No. 3
March 2005
Vol. 41 • No. 3
March 2005
anti-inflammatory agent
Cell line
DNA binding assay
transcription