Translator Disclaimer
1 March 2006 MULTIPLE INITIAL CULTURE CONDITIONS ENHANCE THE ESTABLISHMENT OF CELL LINES FROM PRIMARY OVARIAN CANCER SPECIMENS
CARA C. BERTOZZI, CHENG-YI CHANG, SONYA JAIRAJ, XIAOCHUAN SHAN, JIA HUANG, BARBARA L. WEBER, CHRISTINA S. CHU, RICHARD G. CARROLL
Author Affiliations +
Abstract

To increase the efficiency of stable cell line establishment from primary ovarian cancer specimens, we simultaneously initiated cultures under multiple conditions, varying extracellular matrices and the inclusion of supplements (e.g., serum or serum albumin), while minimizing exposure to xenogeneic antigens (e.g., fetal calf serum). Primary cultures were initiated from 30 specimens; cell lines were established from 10 of these for a success rate of 33%. In some instances, multiple cell lines were established from the same specimen. Five lines were characterized extensively with respect to growth properties, antigen expression, and genomic alterations. Although these lines are all low-passage, marked heterogeneity was observed, even between lines derived from the same specimen. The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology.

CARA C. BERTOZZI, CHENG-YI CHANG, SONYA JAIRAJ, XIAOCHUAN SHAN, JIA HUANG, BARBARA L. WEBER, CHRISTINA S. CHU, and RICHARD G. CARROLL "MULTIPLE INITIAL CULTURE CONDITIONS ENHANCE THE ESTABLISHMENT OF CELL LINES FROM PRIMARY OVARIAN CANCER SPECIMENS," In Vitro Cellular & Developmental Biology - Animal 42(3), 58-62, (1 March 2006). https://doi.org/10.1290/0512084.1
Received: 14 December 2005; Accepted: 21 January 2006; Published: 1 March 2006
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
array comparative genomic hybridization
Cell line
genomic heterogeneity
ovarian cancer
tumor culture
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top