For the first time, regenerated plantlets were obtained from immature zygotic embryos of mango (Mangifera indica L.) through direct somatic embryogenesis. Pro-embryogenic mass (PEM)-like structures, which are differentiated as clusters of globular structures, were easily induced directly from the abaxial side of cotyledons from immature fruits, 2.0–3.5 cm diameter by a 2-wk culture period on a modified Murashige and Skoog medium with 5 mg l−1 (25 μM) indole-3-butyric acid (IBA). Conversion of somatic embryos into plantlets was achieved after 4 wk of culture on the conversion medium containing 5 mg l−1 (23 μM) kinetin. Secondary somatic embryogenesis could also be obtained directly from the hypocotyls of mature primary somatic embryos cultured on the conversion medium. In our experimental system, only minor problems were noted with browning of cultures.
How to translate text using browser tools
1 March 2004
DIRECT SOMATIC EMBRYOGENESIS INDUCED FROM COTYLEDONS OF MANGO IMMATURE ZYGOTIC EMBRYOS
JIE-NING XIAO,
XUE-LIN HUANG,
YONG-JIE WU,
XIAO-JU LI,
MING-DE ZHOU,
FLORENT ENGELMANN
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 2
March 2004
Vol. 40 • No. 2
March 2004
direct somatic embryogenesis
immature cotyledon
Mangifera indica
pro-embryogenic masses
seedling somatic embryogenesis