How to translate text using browser tools
1 November 2005 LOW TEMPERATURE STORAGE OF SUSPENSION CULTURE-DERIVED GRAPEVINE SOMATIC EMBRYOS AND REGENERATION OF PLANTS
S. JAYASANKAR, M. VAN AMAN, J. CORDTS, S. DHEKNEY, Z. T. LI, D. J. GRAY
Author Affiliations +
Abstract

A method of clonal germplasm preservation utilizing dehydrated somatic embryos and cool temperature storage conditions was demonstrated. Somatic embryos of grapevine (Vitis vinifera L.) Autumn Seedless and Chardonnay were produced from suspension cultures. After washing twice with sterile water, mature somatic embryos were blot-dried and placed on sterile filter paper in an open Petri dish in a laminar flow hood until they reached about 25% of their initial weight. Approximately 300 dried embryos were placed in each sterile 90×15 mm Petri dish, which was tightly sealed with two layers of ParafilmTM. Sealed dishes were stored in the dark at 4°C in a standard refrigerator. Samples of 25–60 individual dehydrated somatic embryos were periodically tested for viability by placing them on solidified MS medium for germination and plant regeneration. After 42 mo. of dehydrated storage, 90% of the somatic embryos regenerated into plants. To further test utility of this storage method, dehydrated embryos stored for 12 and 26 mo. were shipped from Florida to Washington where 75 and 87.5% regenerated into plants, respectively. Cool temperature storage of dehydrated somatic embryos is a simple and inexpensive method of clonal germplasm preservation when compared to alternatives such as cryopreservation.

S. JAYASANKAR, M. VAN AMAN, J. CORDTS, S. DHEKNEY, Z. T. LI, and D. J. GRAY "LOW TEMPERATURE STORAGE OF SUSPENSION CULTURE-DERIVED GRAPEVINE SOMATIC EMBRYOS AND REGENERATION OF PLANTS," In Vitro Cellular and Developmental Biology - Plant 41(6), 752-756, (1 November 2005). https://doi.org/10.1079/IVP2005697
Received: 24 January 2005; Accepted: 1 May 2005; Published: 1 November 2005
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

KEYWORDS
somatic embryogenesis
synthetic seeds
Vitis vinifera
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top