In its androgenic response, maize is considered to be a recalcitrant plant. We used mechanically isolated microspores of maize genotype A18 to establish a responsive microspore culture of maize. Morphological events occurring during the first days of maize androgenesis in a microspore culture were observed and described, and some morphological markers for distinguishing between embryogenic microspores and nonembryogenic microspores were identified. It was found that the enlargement of microspores during the first days in culture and the ‘star-like’ organization of the cytoplasm inside the microspore are connected with reprogramming of the developmental pathway in maize microspores. Some differences were also found in the surface wall architecture of embryogenic microspores. Fertile plants were successfully recovered from microspore-originated structures.
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1 November 2005
MORPHOLOGICAL EVENTS IN CULTURES OF MECHANICALLY ISOLATED MAIZE MICROSPORES
BOHUŠ OBERT,
LÁSZLÓ SZABÓ,
JUDIT MITYKÓ,
ANNA PREŤOVÁ,
BEÁTA BARNABÁS
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In Vitro Cellular and Developmental Biology - Plant
Vol. 41 • No. 6
November 2005
Vol. 41 • No. 6
November 2005
in vitro androgenesis
microspore
Zea mays L