This study reports a protocol for germination of Givotia rottleriformis (var. Tel. Thella Poniki) using zygotic embryo culture. A 100% germination was obtained by culturing the embryos on Murashige and Skoog medium containing 30 g l−1 sucrose. A sucrose concentration lower or higher than 30 g l−1 resulted in lower germination or promoted callus formation. The seedling growth was promoted by the addition of 100 mg l−1 tyrosine in the medium. Seedlings germinated in the presence of 0.2–0.4 mg l−1 α-naphthaleneacetic acid and 0.3–0.5 mg l−1 indole-3-butyric acid were abnormal, showing a slender stem with slender roots or forming callus with stout roots. Germination also affected embryo orientation in culture; placing embryos upright on the medium was most beneficial for germination. The in vitro-germinated seedlings were acclimatized in soil under shady conditions with a survival rate of 60–70%. These plants were phenotypically normal, healthy, and similar to donor plants. This protocol will be useful for overcoming seed dormancy and for rapid multiplication and conservation of G. rottleriformis using zygotic embryo culture.
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1 September 2006
IN VITRO ZYGOTIC EMBRYO CULTURE OF AN ENDANGERED FOREST TREE GIVOTIA ROTTLERIFORMIS AND FACTORS AFFECTING ITS GERMINATION AND SEEDLING GROWTH
M. RAMBABU,
M. UPENDER,
D. UJJWALA,
T. UGANDHAR,
M. PRAVEEN,
N. RAMA SWAMY
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 5
September 2006
Vol. 42 • No. 5
September 2006
endangered forest tree
Givotia rottleriformis
in vitro culture
Zygotic embryo