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1 November 2006 PHOTOSYNTHETIC AND CARBOHYDRATE STATUS OF EASY- AND DIFFICULT-TO-ACCLIMATIZE SEA OATS (UNIOLA PANICULATA L.) GENOTYPES DURING IN VITRO CULTURE AND EX VITRO ACCLIMATIZATION
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Abstract

The photosynthetic and carbohydrate status of an easy-to-acclimatize (EK 16-3) and a difficult-to-acclimatize (EK 11-1) genotype of Uniola paniculata L. (sea oats), a native dune species of the southeastern US, were evaluated during in vitro culture and ex vitro acclimatization. Net photosynthetic rate was eight times greater for EK 16-3 than EK 11-1 plantlets after ex vitro transfer. In vitro-produced leaves were morphologically similar to ex vitro-produced leaves and exhibited similar photosynthetic competence. EK 11-1 plantlets exhibited greater transpiration rates at the time of ex vitro transfer than EK 16-3 plantlets. However, the small magnitude of this difference, although significant, indicated that control of water loss was probably not the main cause for poor acclimatization of EK 11-1 plantlets. Carbohydrate analysis in vitro revealed that EK 16-3 plantlets utilized leaf starch reserves more rapidly than EK 11-1 plantlets. Starch utilization correlated with the development of leaves with expanded leaf blades during in vitro rooting in EK 16-3 plantlets. After ex vitro transfer, both genotypes exhibited significant decreases of starch and soluble sugar content in shoots and roots. However, the higher photosynthetic ability of shoots in EK 16-3 resulted in greater accumulation of shoot soluble sugars than EK 11-1 after 2-wk ex vitro culture. After 6-wk in vitro rooting, there were significantly higher chlorophyll and soluble protein contents, ribulose 1,5-bisphosphate carboxylase (rubisco) and phosphoenolpyruvate carboxylase activities in EK 16-3 than EK 11-1 shoots. These differences also correlated with the development of anatomical and morphological leaf features in EK 16-3 similar to those of greenhouse-produced leaves.

CARMEN VALERO-ARACAMA, MICHAEL E. KANE, SANDRA B. WILSON, JOSEPH C. VU, JOAN ANDERSON, and NANCY L. PHILMAN "PHOTOSYNTHETIC AND CARBOHYDRATE STATUS OF EASY- AND DIFFICULT-TO-ACCLIMATIZE SEA OATS (UNIOLA PANICULATA L.) GENOTYPES DURING IN VITRO CULTURE AND EX VITRO ACCLIMATIZATION," In Vitro Cellular and Developmental Biology - Plant 42(6), 572-583, (1 November 2006). https://doi.org/10.1079/IVP2006822
Received: 17 January 2006; Accepted: 21 August 2006; Published: 1 November 2006
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