A 2-year-old domestic hen was presented for a 15-day history of intermittent left leg lameness. The owners also observed a decrease in egg quality during that period. Physical examination of the bird revealed a generalized weakness with a flattened pale comb. Neurological evaluation of the left leg found a proprioceptive deficit with no loss of pain sensitivity. Whole body radiographic images showed a marked reduction of abdominal detail, a large amount of “grit” (mineral opacity) in the ventriculus, intestinal dilation, and a metallic foreign body (nail) in the digestive tract. Abnormal results from a plasma biochemistry panel were hypercalcemia, hypertriglyceridemia, hyperglobulinemia, hypophosphatemia, increased creatine kinase, and decreased uric acid. After initial stabilization of the patient, a computed tomographic scan was obtained, which revealed multiple right-sided ureteral calculi, the largest of which measured 10 mm in diameter, and changes compatible with cystic right renal nephrosis. Suspected compensatory enlargement of the left kidney was also observed. Considering the renal lesion and associated neurological limb impairment, surgery was recommended and approved by the owner. A right lateral approach was used for the coeliotomy, and a ureterotomy was performed to remove all calculi. Analysis of the ureteral stones confirmed they were 100% uric acid salts. The hen fully recovered left leg function 1 week postsurgery. Urolithiasis is well described in hens, but surgical treatment is uncommon. This report describes the successful removal of ureteral stones by ureterotomy in a domestic hen.