Drosophila suzukii Matsumura (Diptera: Drosophilidae) has caused significant economic damage to berry and stone fruit production regions. Markers that are systemic in plants and easily transferred to target organisms are needed to track D. suzukii exploitation of host resources and trophic interactions. High and low concentrations of the trace element, rubidium (Rb), and the stable isotope, ¹⁵N, were tested to mark D. suzukii larvae feeding on fruits of enriched strawberry plants grown in containers under greenhouse conditions. Fly marker content and proportion of flies marked 1, 7, and 14 d after emergence from enriched fruits and fly dry mass were analyzed. Nearly 100% of the flies analyzed 14 d after emerging from ¹⁵N-enriched plants were marked, whereas only 30–75% and 0–3% were marked 14 d after emerging from high and low Rb concentration plants, respectively. Rapid Rb decay, strong ¹⁵N persistence, and the economics of using these markers in the field to elucidate D. suzukii pest ecology are discussed.