Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) attacks a wide range of host plants, including crops such as blueberries, cherries, caneberries, and strawberries. We evaluated the influence of three temperatures (1.1, 3.9, and 5.0 °C) and four exposure durations (6, 12, 24, and 72 h) that were selected to represent typical grower practices on survival and development time of immature D. suzukii in artificial diet and one temperature and duration (1.67 °C for 72 h) in fruit (blueberries and raspberries). Cold storage at 1.1 °C for 24 h decreased larval survival, but shorter durations generally did not affect larval survival. No eggs or young larvae survived when held for 72 h at 1.1 °C, and fewer older larvae survived after 72 h at 1.1, 3.9, and 5.0 °C than at 20 °C (control). Development time in diet was longer for all life stages following at least 12 h at any of the storage temperatures. In blueberries, no eggs survived to pupation when stored at 1.67 °C for 72 h, and survival of third instars was reduced by 41%. In raspberries, egg, second instar, and third instar survival was significantly reduced following storage at 1.67 °C for 72 h. Drosophila suzukii larval development time was shorter in raspberry than in blueberry, but it was significantly longer in both fruits when stored at 1.67 °C for 72 h. Our results indicate that cold storage can reduce survival and increase development time of immature D. suzukii, and it could be a useful part of an integrated program to manage D. suzukii infestation.