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3 April 2018 Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae)
Yueliang Zhang, Baosheng Liu, Zhichun Zhang, Lihua Wang, Huifang Guo, Zhong Li, Peng He, Zewen Liu, Jichao Fang
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Abstract

Imidacloprid is a key insecticide used for controlling sucking insect pests, including the small brown planthopper (Laodelphax striatellus, Fallén) (Hemiptera: Delphacidae), an important agricultural pest of rice. A strain of L. striatellus (YN-ILR) developed 21-fold resistance when selected with imidacloprid on a susceptible YN strain. An in vitro study on piperonyl butoxide synergism indicated that enhanced detoxification mediated by cytochrome P450s contributed to imidacloprid resistance to some extent, and multiple P450 genes showed altered expression in the imidacloprid-resistantYN-ILR strain compared with the susceptibleYN strain (CYP425B1-CYP6BD10 had 1.51- to 11.45-fold higher expression, CYP4CE2-CYP4DD1V2 had 0.12- to 0.57-fold lower expression). While there were no mutations in target nicotinic acetylcholine receptor (nAChR) genes, subunits of Lsα1, Lsβ1, and Lsβ3 in the YN-ILR strain showed 3.86-, 4.39-, and 2.59-fold higher expression and Lsa8 displayed 0.38-fold lower expression than the YN strain. Moreover, 21-fold moderate imidacloprid resistance in individuals of L. striatellus did not produce a fitness cost. The findings suggest that L. striatellus has the capacity to develop resistance to imidacloprid through P450 detoxification and potential target nAChR expression changes, and moderate imidacloprid resistance was not associated with a fitness cost.

© The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Yueliang Zhang, Baosheng Liu, Zhichun Zhang, Lihua Wang, Huifang Guo, Zhong Li, Peng He, Zewen Liu, and Jichao Fang "Differential Expression of P450 Genes and nAChR Subunits Associated With Imidacloprid Resistance in Laodelphax striatellus (Hemiptera: Delphacidae)," Journal of Economic Entomology 111(3), 1382-1387, (3 April 2018). https://doi.org/10.1093/jee/toy051
Received: 25 September 2017; Accepted: 3 January 2018; Published: 3 April 2018
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KEYWORDS
Cytochrome P450s
fitness cost
imidacloprid
Laodelphax striatellus
nicotinic acetylcholine receptor
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