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8 December 2018 Alternative Splicing of the CpomOR53 Gene Produces Three Transcripts Expressed in Codling Moth (Lepidoptera: Tortricidae) Antennae
Stephen F. Garczynski, Alberto M. Cattaneo, William B. Walker III
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Abstract

Incorporation of semiochemicals into codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), IPM programs has drastically reduced the amount of chemical insecticides needed to control this orchard pest. Odorant receptors are key sensors in the detection of semiochemicals and trigger downstream signaling events leading to behavioral responses. For codling moth, 58 odorant receptors have been identified in antennal transcriptomes, a few of which have been characterized for ligand activation. From the codling moth antennal transcriptome, a single transcript encoding CpomOR53 was annotated but re-evaluation suggests two or more variants of this receptor may be present and it is hypothesized that they are produced by alternative splicing. In this study, the complete open reading frame of CpomOR53 was amplified from codling moth male and female antennal RNAs, with three distinct transcripts detected. Characterization of these transcripts indicate that they are produced by alternative splicing of the CpomOR53 gene. The membrane topology for each of the CpomOR53 variants shows that alternative spliced products altered the length of intracellular loop two of the predicted proteins. The effects of these alterations were not determined but will be addressed in future studies determining the ligand(s) that activate each CpomOR53 transcript variant.

Published by Oxford University Press on behalf of Entomological Society of America 2018. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Stephen F. Garczynski, Alberto M. Cattaneo, and William B. Walker III "Alternative Splicing of the CpomOR53 Gene Produces Three Transcripts Expressed in Codling Moth (Lepidoptera: Tortricidae) Antennae," Journal of Economic Entomology 112(2), 991-996, (8 December 2018). https://doi.org/10.1093/jee/toy370
Received: 25 September 2018; Accepted: 5 November 2018; Published: 8 December 2018
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KEYWORDS
alternative splicing
antenna transcript
odorant receptor
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