Incorporation of semiochemicals into codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), IPM programs has drastically reduced the amount of chemical insecticides needed to control this orchard pest. Odorant receptors are key sensors in the detection of semiochemicals and trigger downstream signaling events leading to behavioral responses. For codling moth, 58 odorant receptors have been identified in antennal transcriptomes, a few of which have been characterized for ligand activation. From the codling moth antennal transcriptome, a single transcript encoding CpomOR53 was annotated but re-evaluation suggests two or more variants of this receptor may be present and it is hypothesized that they are produced by alternative splicing. In this study, the complete open reading frame of CpomOR53 was amplified from codling moth male and female antennal RNAs, with three distinct transcripts detected. Characterization of these transcripts indicate that they are produced by alternative splicing of the CpomOR53 gene. The membrane topology for each of the CpomOR53 variants shows that alternative spliced products altered the length of intracellular loop two of the predicted proteins. The effects of these alterations were not determined but will be addressed in future studies determining the ligand(s) that activate each CpomOR53 transcript variant.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 112 • No. 2