The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a destructive insect pest of cruciferous plants that has developed resistance to almost every listed commercial insecticide. Cantharidin as an animal-derived biopesticide is a natural defensive compound produced by Meloidae insects with toxicity to many insects including P. xylostella. Norcantharidin is an important substitute of cantharidin and has similar insecticidal activities to cantharidin. Although the toxicity of cantharidin and norcantharidin to P. xylostella has been reported, little research has focused on the effects of cantharidin or norcantharidin on the behavior of P. xylostella. In this study, we investigated the feeding behavior of third-instar larvae and the oviposition preference of adult P. xylostella in order to explore the effects of different concentrations of cantharidin and norcantharidin. Results show that cantharidin and norcantharidin have antifeedant effect on P. xylostella larvae. The values for AFC₅₀ were 13.0228 and 149.4210 mg/ml, respectively. Furthermore, the oviposition deterrence rate of cantharidin on P. xylostella ranged from 49.37 to 58.24% and that of norcantharidin was from 20.88 to 33.33%. These results suggest cantharidin and norcantharidin may have repellent and antifeedant effect on P. xylostella, which could contribute toward using biopesticides to manage P. xylostella and may provide a new strategy for integrated pest management.