New formulations of formic acid and thymol, both individually and in combination with various essential oils, were compared with Apistan to determine their efficacy as fall treatments for control of V arroa jacobsoni (Oudemans), a parasitic mite of the honey bee, Apis mellifera L. Percent mite mortality in colonies treated with 300 ml of 65% formic acid averaged 94.2 ± 1.41% (least square means ± SE, n = 24), equivalent to those receiving four, 10% strips of Apistan (92.6 ± 1.79%, n = 6). Treatment with thymol ( n = 24) resulted in an average mite mortality of 75.4 ± 5.79%, significantly less than that attained with Apistan or formic acid. The addition of essential oils did not affect treatment efficacy of either formic acid or thymol. The ratio of the coefficients of variation for percentage mortality for the formic acid (CV_FA) and Apistan (CV_A) groups was CV_FA/CV_A = 0.66. This indicates that the formic acid treatment was as consistent as the Apistan treatment. Thymol treatments did not provide as consistent results as Apistan or formic acid. Coefficient variation ratios for percentage mortality for the thymol group (CV_T) with the Apistan and formic acid groups were CV_T/CV_A = 4.47 and CV_T/CV_FA = 6.76, respectively. In a second experiment, colonies received a 4-wk fall treatment of either 300 ml of 65% formic acid ( n = 24) or four, 10% strips of Apistan ( n = 6). The next spring, mite levels in the formic acid group (554.3 ± 150.20 mites) were similar to those in the Apistan treatment group (571.3 ± 145.05 mites) ( P = 0.93). Additionally, the quantities of bees, brood, pollen, and nectar/honey in the two treatment groups were not significantly different ( P ≥ 0.50 each variable). These results suggest that formic acid is an effective alternative to Apistan as a fall treatment for varroa mites in temperate climates.