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1 June 2002 Binary Insecticidal Crystal Protein from Bacillus thuringiensis, Strain PS149B1: Effects of Individual Protein Components and Mixtures in Laboratory Bioassays
Rod A. Herman, Peter N. Scherer, Debra L. Young, Charles A. Mihaliak, Thomas Meade, Aaron T. Woodsworth, Brian A. Stockhoff, Kenneth E. Narva
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Abstract

A family of novel binary insecticidal crystal proteins, with activity against western corn rootworm, Diabrotica virgifera virgifera LeConte, was identified from Bacillus thuringiensis Berliner. A binary insecticidal crystal protein (bICP) from B. thuringiensis strain PS149B1 is composed of a 14-kDa protein (Cry34Ab1) and a 44-kDa protein (Cry35Ab1). These proteins have been co-expressed in transgenic maize plants, Zea mays L., and effectively control western corn rootworm larvae under field conditions. Laboratory experiments were conducted to better understand the contribution of each component protein to the in vivo activity of the bICP. The 14-kDa protein is active alone against southern corn rootworm, Diabrotica undecimpunctata howardi Barber, and was synergized by the 44-kDa protein. In mixtures, the concentration of the 14-kDa protein had a greater impact on efficacy than the 44-kDa component. Although both proteins are clearly required for maximal insecticidal activity, laboratory results did not support the formation of a stable, fixed-ratio complex of the two component proteins.

Rod A. Herman, Peter N. Scherer, Debra L. Young, Charles A. Mihaliak, Thomas Meade, Aaron T. Woodsworth, Brian A. Stockhoff, and Kenneth E. Narva "Binary Insecticidal Crystal Protein from Bacillus thuringiensis, Strain PS149B1: Effects of Individual Protein Components and Mixtures in Laboratory Bioassays," Journal of Economic Entomology 95(3), 635-639, (1 June 2002). https://doi.org/10.1603/0022-0493-95.3.635
Received: 26 July 2001; Accepted: 1 February 2002; Published: 1 June 2002
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