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1 December 2005 Starch Industry Wastewater-Based Stable Bacillus thuringiensis Liquid Formulations
Satinder K. Brar, M. Verma, R. D. Tyagi, J. R. Valéro, R. Y. Surampalli
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Abstract

Liquid formulations were developed from Bacillus thuringiensis (Bt)-fermented broths of starch industry wastewater (SIW) and of soya medium. Stability studies were carried out for 1 yr. Storage stability was tested by studying various physical and chemical (e.g., viscosity, particle size, corrosion, and suspendibility) and biological (e.g., microbial contamination, viable spores, and entomotoxicity) parameters at different pH levels and temperatures. Three suspending agents, sorbitol, sodium monophosphate, and sodium metabisulfite, were added to fermented broth in different concentrations. Sorbitol and sodium monophosphate in the ratio 3:1 was the best suspending agent combination for both formulations. Starch industry wastewater fermentation yielded cell and viable spore counts 10- and 4-fold greater than those from soya medium, respectively, and a 1.7-fold increase in entomotoxicity. However, both formulations started deteriorating at pH 6 and 6.5 and 40 and 50°C. There were no signs of corrosion and microbial contamination in both types of formulations.

Satinder K. Brar, M. Verma, R. D. Tyagi, J. R. Valéro, and R. Y. Surampalli "Starch Industry Wastewater-Based Stable Bacillus thuringiensis Liquid Formulations," Journal of Economic Entomology 98(6), 1890-1898, (1 December 2005). https://doi.org/10.1603/0022-0493-98.6.1890
Received: 5 June 2005; Accepted: 1 August 2005; Published: 1 December 2005
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