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1 February 2006 Effects of Long-Term Storage on Potency of TM Biocontrol-1, the Registered Viral Insecticide of Orgyia pseudotsugata (Lepidoptera: Lymantriidae)
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Abstract

The article presents the results of bioassaying 39 samples of TM Biocontrol-1, a viral insecticide, from 10 different lots and various sizes of vacuum-sealed packages that were stored at −10°C for 5–15 yr. This is the first study to present potency data for a registered virus product stored for this length of time. Laboratory bioassays in insects from the same colony from which the TM Biocontrol-1 was produced showed that the stored viral insecticide is still effective, although it lost ≈30% of its effectiveness during storage. A direct correlation of this loss with the length of time in storage is not apparent. Bioassays also showed that there are significant differences in the susceptibility of Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough), larvae from different geographic regions to OpMNPV (family Baculoviridae, genus Nucleopolyhedrovirus) infection. Package size did not affect the potency of stored TM Biocontrol-1. There were no clear, significant differences in the effectiveness among lots of TM Biocontrol-1 processed by different organizations.

I. S. Otvos, B. Kukan, R. Reardon, and I. Ragenovich "Effects of Long-Term Storage on Potency of TM Biocontrol-1, the Registered Viral Insecticide of Orgyia pseudotsugata (Lepidoptera: Lymantriidae)," Journal of Economic Entomology 99(1), 14-22, (1 February 2006). https://doi.org/10.1603/0022-0493(2006)099[0014:EOLSOP]2.0.CO;2
Received: 29 November 2004; Accepted: 12 September 2005; Published: 1 February 2006
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