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1 October 2006 Partial Purification and Insecticidal Activity of Toxic Metabolites Secreted by a Mucor hiemalis Strain (SMU-21) Against Adults of Bactrocera oleae and Ceratitis capitata (Diptera: Tephritidae)
M. A. Konstantopoulou, P. Milonas, B. E. Mazomenos
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Abstract

The secondary metabolites present in the methanol extract of a Mucor hiemalis strain (SMU-21) mycelia, cultured in liquid medium, were evaluated for toxicity to Bactrocera oleae (Gmelin) and Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) adults. Feeding and contact bioassays revealed that the methanol fraction of the crude supernatant was strongly toxic to both species. Symptoms of toxicity developed quickly; lethargy occurred 1–2 h posttreatment, and mortality reached 82–97% after 24 h. Both feeding and contact bioassays showed that B. oleae was more susceptible than C. capitata. Concentrations producing 50% mortality (LC50) to B. oleae and C. capitata after 24 h in feeding bioassays were 0.52 and 1.28 mg/ 0.1 ml diet, respectively, but 34.8 and 64.0 μg/ cm2, respectively, after 4 h in contact bioassays. Solvent partition, precipitation, and chromatographic procedures were used to isolate the active principles from the crude supernatant. These resulted in the isolation of one high-pressure liquid chromatography fraction with insecticidal activity on B. oleae flies equal to the initial crude supernatant.

M. A. Konstantopoulou, P. Milonas, and B. E. Mazomenos "Partial Purification and Insecticidal Activity of Toxic Metabolites Secreted by a Mucor hiemalis Strain (SMU-21) Against Adults of Bactrocera oleae and Ceratitis capitata (Diptera: Tephritidae)," Journal of Economic Entomology 99(5), 1657-1664, (1 October 2006). https://doi.org/10.1603/0022-0493-99.5.1657
Received: 10 January 2006; Accepted: 1 June 2006; Published: 1 October 2006
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KEYWORDS
acute activity
fungal extract
microbial pesticides
Mucorales
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