Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) has been phylogenetically divided into several putative species based on nucleotide sequence of mitochondrial cytochrome oxidase 1 (mtCOI). To confirm the putative species among B. tabaci populations in Iran, insects were collected from different regions and plant hosts in Khuzestan province of southwestern Iran, and their mtCOI sequences were determined. DNA polymorphism and phylogenetic analysis of the 10 mtCOI sequences obtained showed four haplotypes among the specimens from Khuzestan province that were identified as the putative species MEAM1-subcladeB. Moreover, specimens previously collected from Iran were found to be members of the MEAM1-subcladeB2. Twelve variable sites were detected throughout the Iran-originated mtCOI sequences contributing to their position on the phylogenetic tree. This is the first study reporting the putative species MEAM1-subcladeB from southwestern Iran.