Tonometry, the estimation of intraocular pressure (IOP), is an essential component of ophthalmic examination. Understanding of this diagnostic tool is limited in chelonians. The purpose of this study was to evaluate two methods of tonometry for estimation of IOP in red-eared slider turtles (Trachemys scripta elegans) and report values for corneal sensitivity measured by corneal aesthesiometry. Adult redeared slider turtles (n = 6) were manually restrained for tonometry; rebound and applanation tonometers were used. Corneal aesthesiometry was performed with the use of a Cochet-Bonnet corneal aesthesiometer. Median IOP estimated with applanation tonometry was 31.5 mmHg (25–75%: 24.3–38.3 mmHg, min–max: 9–58 mmHg) OD and 23.5 mmHg (25–75%: 17.8-34.8 mmHg, min–max: 10–58 mmHg) OS. Mean IOP estimated with rebound tonometry with non–species-specific calibration was 6.1 mmHg (SD: 2.3 mmHg, min–max: 3–9 mmHg) OD and 5.7 mmHg (SD: 1.3 mmHg, min–max: 2–9 mmHg) OS, with canine-specific calibration it was 9.2 mmHg (SD: 1.8 mmHg, min–max: 5–14mmHg) OD and 10.2 mmHg (SD: 1.5 mmHg, min–max: 7–12 mmHg) OS, and with equine-specific calibration it was 9.1 mmHg (SD: 0.9 mmHg, min– max: 8–13 mmHg) OD and 8.4 mmHg (SD: 1.2 mmHg, min–max: 5–11 mmHg) OS. There was poor level of agreement between applanation and rebound tonometers. Median corneal touch threshold was 6 cm (25–75%: 4.5–6 cm, min–max 0.5–6.0 cm). Results show that red-eared slider turtles have high corneal sensitivity and that both applanation and rebound tonometry are feasible in this species; however, more precision was noted with the rebound tonometer. Collecting serial measurements with the use of the same device is recommended to limit the potential for misclassification bias.