Thirty-four eastern indigo snakes (Drymarchon couperi) naturally infected with Cryptosporidium serpentis were randomly divided into two groups. The first group received 360 mg/kg paromomycin twice weekly in a food item for 6 wk, and the second group received the food item with no treatment. Cloacal swabs were collected every 2 months for 6 months to measure C. serpentis shedding by probe hybridization quantitative polymerase chain reaction testing (qPCR). Snakes that were qPCR negative after 6 months were immunosuppressed with a single dose of 4 mg/kg dexamethasone sodium-phosphate SC. These snakes were then screened by qPCR for an additional 6 months as described above. Snakes that were qPCR negative after 1 yr of serial sampling were then re-evaluated for C. serpentis via gastric biopsy for histological and qPCR analyses. The paromomycin-treated group were significantly (P = 0.008) more likely to test qPCR negative (8/17; 47%, 95% confidence interval [CI]: 23.2–70.7) than the control snakes (1/17; 5.8%, 95% CI: 0.01–16.9) prior to immunosuppression. However, there was no significant difference (P = 0.5) in C. serpentis status following immunosuppression, as only 2/17 (11.7%, 95% CI: 0.01–26.9) paromomycin-treated snakes were qPCR negative 6 months after immunosuppression compared to 1/17 (5.8%, 95% CI: 95% CI: 0.01–16.9) control snakes. These findings suggest that 360 mg/kg paromomycin twice weekly for 6 wk in a food item is ineffective in eliminating C. serpentis in naturally infected D. couperi.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.