Knowledge of habitat preferences by an invasive species provides a theoretical background for habitat management decisions. We used stepwise logistic regression to develop probabilistic models predicting the presence or absence of the postmetamorphic American Bullfrog (Lithobates catesbeianus) on Daishan Island. We found American Bullfrogs in 38 of 102 searched quadrats. Modeling indicated American Bullfrogs preferred permanent still water, such as large reservoirs and ponds. There was also a negative correlation between the presence of American Bullfrogs and the abundance of native frogs, suggesting that American Bullfrogs either represent a threat to native frogs or preferentially colonize sites where native frogs are rare or absent. This study provides a foundation on which to develop initial strategies to manage American Bullfrogs on the island and to assist in forecasting the areas suitable for invasion by American Bullfrogs in the region.