Female age influence on mating success, courtship activities, mating latency, copulation duration, fecundity, ovarioles number, and wing length has been studied using isofemale lines of Drosophila bipectinata collected at three different localities. It was observed that in all localities, middle-aged D. bipectinata females had significantly greater mating success, showed less rejection responses to courting male, mated faster, copulated longer, and had greater fecundity and ovariole number than young and old-aged females. Further, old-aged females had comparatively less fitness traits than young age females. This research suggests the occurrence of age specific female reproductive success as follows: middle-aged > young > old-aged.