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1 June 2013 Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
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Abstract

The secret cave cricket, Ceuthophilus secretes Scudder (Orthroptera: Rhaphidophoridae), is an obligate trogloxene endemic to central Texas, USA, and is a primary source of energy and nutrients for sensitive cave ecosystems. In this study, nine polymorphic microsatellite markers were developed from genomic DNA of C. secretes. Genotypes of 120 individuals sampled from four localities on the Fort Hood Military Reservation, Killeen, Texas, were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 19 to 46. All paired loci were in linkage equilibrium. Four loci had significant deviations from Hardy-Weinberg equilibrium. Observed heterozygosity varied from 0.793 to 0.959. These loci provide a means of characterizing population genetic structure in this species.

This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
Nichole Hutchison, Paul. L. Leberg, and Richard F. Lance "Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus," Journal of Insect Science 13(64), 1-6, (1 June 2013). https://doi.org/10.1673/031.013.6401
Received: 13 January 2011; Accepted: 1 May 2012; Published: 1 June 2013
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