Translator Disclaimer
1 April 2007 The Movement of Water and Cryoprotectants in Mammalian Oocytes and Embryos and its Relevance to Cryopreservation
Keisuke Edashige, Magosaburo Kasai
Author Affiliations +
Abstract

The conditions suitable for cryopreservation differ among oocytes and embryos at different stages even in the same species. Differences in cryobiological properties would affect these conditions. Permeability to water and cryoprotectants is important because it modulates major forms of cell injury from cryopreservation. In the mouse, morulae and blastocysts tolerate cryopreservation better than oocytes or early stage embryos, because they are highly permeable to water and cryoprotectants. The permeability appears to stem from a substantial expression of water/cryoprotectant-permeable channels, such as aquaporin-3. Such facilitated diffusion by channel proteins is less dependent on temperature than the process of simple diffusion via the lipid bilayer. Therefore, it is important to know the expression level and permeation properties of water/cryoprotectant channels for efficient cryopreservation of oocytes and embryos.

Keisuke Edashige and Magosaburo Kasai "The Movement of Water and Cryoprotectants in Mammalian Oocytes and Embryos and its Relevance to Cryopreservation," Journal of Mammalian Ova Research 24(1), 18-22, (1 April 2007). https://doi.org/10.1274/jmor.24.18
Received: 8 February 2007; Accepted: 1 February 2007; Published: 1 April 2007
JOURNAL ARTICLE
5 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top