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1 April 2009 Transition of Cleavage Divisions During In Vitro Development of Bovine Embryos
Hitoshi Ushijima, Kiyoshi Akiyama, Toshio Tajima
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Abstract

Bovine in vitro fertilized (IVF) embryos were cultured in either CR1aa (CR1) or TCM-199 (TCM) medium and compared for their daily development based on the number of cleavage divisions (CDN), as calculated from the total cell numbers of the embryos. Embryos with advanced developmental stage and higher morphological quality were selected for use in the experiment. The relation between the CDN and the number of embryonic days after IVF in both groups showed a linear correlation; no significant difference (P > 0.05) was found between the two groups. However, CDN on days 3 and 8 after IVF in the CR1 group did not increase, which suggests that transient developmental arrests occurred at these stages. In contrast, embryos in the TCM group showed a transient evelopmental arrest 3 d after IVF, but CDN increased regularly with age in days at later stages of culture with 100 µM βmercaptoethanol. A significant difference was found between the regression lines of the two groups during 5–9 d after IVF (P < 0.001). Consequently, numerical analysis of embryonic development in terms of CDN enabled objective evaluation of the developmental progress of bovine IVF embryos.

Hitoshi Ushijima, Kiyoshi Akiyama, and Toshio Tajima "Transition of Cleavage Divisions During In Vitro Development of Bovine Embryos," Journal of Mammalian Ova Research 26(1), 42-47, (1 April 2009). https://doi.org/10.1274/jmor.26.42
Received: 2 July 2008; Accepted: 1 October 2008; Published: 1 April 2009
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KEYWORDS
Bovine IVF embryo
Cleavage division
embryonic development
β-mercaptoethanol
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