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1 April 2009 Follicular Loss of the Cryopreserved Canine Ovary after Xenotransplantation
Takako Ishijima, Yasuyuki Abe, Hiroshi Suzuki
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Abstract

The effect of cryopreservation and subsequent xenotransplantation on the follicular reserve of the canine ovary by using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed into the ovarian bursa of mice, and then were removed and subjected to histological examination at 4 weeks after the transplantation. Over 30% of primordial follicles and 65% of early primary follicles survived after cryopreservation. However, regardless of breed or age, percentages of survived primordial follicles and early primary follicles after the transplantation ranged from 0–7% and from 0–15%, respectively. These results indicate that the majority of primordial follicles and early primary follicles in vitrified-warmed canine ovarian tissues disappear after xenotransplantation. Further studies will be required to be able to enhance the survival of transplanted cryopreserved ovarian follicles in canine.

Takako Ishijima, Yasuyuki Abe, and Hiroshi Suzuki "Follicular Loss of the Cryopreserved Canine Ovary after Xenotransplantation," Journal of Mammalian Ova Research 26(1), 61-65, (1 April 2009). https://doi.org/10.1274/jmor.26.61
Received: 10 September 2008; Accepted: 1 December 2008; Published: 1 April 2009
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KEYWORDS
canine
cryopreservation
ovary
Transplantation
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