Daily activity is an important aspect of animal behavior and depends both on nutritional and reproductive demands. It also can be modified by—among other factors—interspecific competition, the need to minimize the risk of predation, and human disturbance, such as the presence of cattle. We studied the activity patterns and degree of overlap among carnivores, their potential wild prey, and livestock, in a Biosphere Reserve in the Chihuahuan Desert. There was no temporal segregation among the carnivores. Carnivores synchronized their activity with that of their main prey, the black-tailed jackrabbit (Lepus californicus). In contrast, we did observe temporal segregation between the puma (Puma concolor), coyote (Canis latrans), and bobcat (Lynx rufus), with mule deer (Odocoileus hemionus). There was little activity overlap between predators and livestock (Bos taurus, Equus caballus). However, to avoid conflict resulting from livestock predation we suggest increasing and improving surveillance, and that calves and females close to calving be sheltered. More studies on the multiniche interactions of livestock and their wild predators are needed to understand daily activity overlap in different seasons, and to better understand the mechanisms of coexistence in protected areas in order to make sound management recommendations to cattle growers and park rangers.