Evolutionary constraints and ecological pressures influence species' morphological diversity. The aim of this study was to explore patterns of morphological variation, to investigate the influence of environmental variables on morphological differentiation, and assess the strength of phylogenetic signal for the cranium and mandible in spiny rats of the genus Trinomys. We examined 377 crania and mandibles of all species of the genus Trinomys. We used geometric morphometric methods based on two-dimensional anatomical landmarks to describe cranium and mandible shape and size. Phylogenetic signal was tested using the K statistics and associations between morphology and environmental variables were made using a phylogenetic partial least squares analysis. We found marked differences in size and shape in the synchranium among species, especially in the segregation of T. albispinus and T. yonenagae from the other species, which in turn greatly overlapped both in size and shape spaces. We found a weak phylogenetic signal for size of the synchranium and intermediate phylogenetic signals for shape, a pattern similar to other studies. Differentiation of the synchranium shape in Trinomys was associated largely with precipitation-related variables, while the association between size and environmental variables was weak. Notably, Trinomys species inhabiting xeric environments had relatively larger tympanic bullae.