Subsampling is used to process scat collections in diet and pollination studies involving nectarivores, but what constitutes a sufficient sampling effort is undetermined. We analyzed entire scat samples from 3 bat species (Pteropodidae) and recorded all pollen; we then tested various levels of subsampling to determine the most efficient regimes. Secondary subsampling (5 fields of view) was inappropriate for plants with low pollen loads (rare pollen); however, abundant pollen morphospecies were detected reliably with little effort. Similarly, the rarer the pollen, the higher was the chance of missing pollen in primary subsampling. Low subsampling effectiveness occurred for scats with high incidences of singletons (= rare pollen); they required the greatest effort to identify ≥ 80% of pollen morphospecies. Diet homogeneity varied among bat species, and subsample efforts should relate to diet strategy and ecology of target species. We recommend using a standard volume of material per subsample; avoiding secondary subsampling unless screening for abundant pollen morphospecies; analyzing fully 20% of material if ≤ 80% of pollen morphospecies need to be detected; and increasing subsampling effort for studies targeting plants with low pollen loads. Subsampling effort needs to match study objectives, or flower species will be greatly underrepresented or missed completely.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 94 • No. 6