Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) Embryos

Dennis R. Berkebile; Jan Chirico; Roger A. Leopold

doi:10.1603/0022-2585-37.6.968

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1 November 2000 Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) Embryos

Dennis R. Berkebile, Jan Chirico, Roger A. Leopold

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J. of Medical Entomology, 37(6):968-972 (2000). https://doi.org/10.1603/0022-2585-37.6.968

Abstract

Embryos of the primary screwworm, Cochliomyia hominivorax (Coquerel), were successfully permeabilized for use in subsequent cryopreservation studies. Mortality was greater for eggs incubated for <5 h before treatment. The mean survival of embryos to first instars was 55.7, 61.1, and 62.6% when the embryos were incubated for 5, 5.5, and 6 h before treatment, respectively. The survival to the pupal and adult stages was low. An improved media for culturing the embryos during and immediately after treatment needs to be devised and the procedure for rearing the larval stages also needs to be altered to improve survival for emerging adults.

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Dennis R. Berkebile, Jan Chirico, and Roger A. Leopold "Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) Embryos," Journal of Medical Entomology 37(6), 968-972, (1 November 2000). https://doi.org/10.1603/0022-2585-37.6.968

Received: 11 January 2000; Accepted: 1 June 2000; Published: 1 November 2000

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