Antimite activity of essential oils and their components obtained from Taiwania cryptomerioides Hayata heartwood against Dermatophagoides pteronyssinus (Trouessart) and Dermatophagoides farinae Hughes was investigated in this study. Results from antimite tests demonstrated that the essential oil extracted from T. cryptomerioides heartwood had miticidal activity against D. pteronyssinus and D. farinae with a mortality of 67.0 and 36.7% at the dosage of 12.6 μg/cm² after 48 h. Alpha-cadinol possessed the strongest antimite activity compared with other components of the T. cryptomerioides heartwood essential oil. The rectified mortalities of D. pteronyssinus and D. farinae were 100% for α-cadinol at the dosage of 6.3 μg/cm². The order of antimite activity of four dominant constituents was α-cadinol > T-muurolol > ferruginol > T-cadinol. Paired Student’s t-tests showed that there were significant differences between the rectified mortality of α-cadinol, T-muurolol, ferruginol and that of T-cadinol at the dosage of 6.3 μg/cm² after 48 h.