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1 May 2007 Different Amino-Acid Substitutions Confer Insecticide Resistance Through Acetylcholinesterase 1 Insensitivity in Culex vishnui and Culex tritaeniorhynchus (Diptera: Culicidae) from China
Haoues Alout, Arnaud Berthomieu, Feng Cui, Yi Tan, Claire Berticat, Chuanling Qiao, Mylène Weill
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Abstract

Insecticide resistance owing to insensitive acetylcholinesterase (AChE)1 has been reported in several mosquito species, and only two mutations in the ace-1 gene have been implicated in resistance: 119S and 331W substitutions. We analyzed the AChE1 resistance status of Culex vishnui (Theobald) and Culex tritaeniorhynchus Giles sampled in various regions of China. These two species displayed distinct mutations leading to AChE1 insensitivity; the 119S substitution in resistant C. vishnui mosquitoes and the 331W substitution in resistant C. tritaeniorhynchus. A biochemical test was validated to detect the 331W mutation in field samples. The comparison of the recombinant G119S and 331W mutant proteins produced in vitro with the AChE1 extracted from resistant mosquitoes indicated that the AChE1 insensitivity observed could be specifically attributed to these substitutions. Comparison of their biochemical characteristics indicated that the resistance conferred by these mutations depends on the insecticide used, regardless of its class. This resistance seemed to be fixed in the Cx. tritaeniorhynchus populations sampled in a 2,000-km transect, suggesting a very high level of insecticide application or a low fitness cost associated with this 331W mutation.

Haoues Alout, Arnaud Berthomieu, Feng Cui, Yi Tan, Claire Berticat, Chuanling Qiao, and Mylène Weill "Different Amino-Acid Substitutions Confer Insecticide Resistance Through Acetylcholinesterase 1 Insensitivity in Culex vishnui and Culex tritaeniorhynchus (Diptera: Culicidae) from China," Journal of Medical Entomology 44(3), 463-469, (1 May 2007). https://doi.org/10.1603/0022-2585(2007)44[463:DASCIR]2.0.CO;2
Received: 9 May 2006; Accepted: 9 August 2006; Published: 1 May 2007
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KEYWORDS
acetylcholinesterase insensitivity
Culex pipiens
Culex tritaeniorhynchus
insecticide resistance
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