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1 July 2009 Genomic Sequence and Phylogenetic Analysis of Culex Flavivirus, an Insect-Specific Flavivirus, Isolated From Culex pipiens (Diptera: Culicidae) in Iowa
Bradley J. Blitvich, Ming Lin, Karin S. Dorman, Victor Soto, Einat Hovav, Bradley J. Tucker, Molly Staley, Kenneth B. Platt, Lyric C. Bartholomay
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Abstract

Adult mosquitoes (Diptera: Culicidae) were collected in 2007 and tested for specific viruses, including West Nile virus, as part of the ongoing arbovirus surveillance efforts in the state of Iowa. A subset of these mosquitoes (6,061 individuals in 340 pools) was further tested by reverse transcription-polymerase chain reaction (RT-PCR) using flavivirus universal primers. Of the 211 pools of Culex pipiens (L.) tested, 50 were positive. One of 51 pools of Culex tarsalis Coquillet was also positive. The flavivirus minimum infection rates (expressed as the number of positive mosquito pools per 1,000 mosquitoes tested) for Cx. pipiens and Cx. tarsalis were 10.3 and 1.2, respectively. Flavivirus RNA was not detected in Aedes triseriatus (Say) (52 pools), Culex erraticus (Dyar & Knab) (25 pools), or Culex territans Walker (one pool). Sequence analysis of all RT-PCR products revealed that the mosquitoes had been infected with Culex flavivirus (CxFV), an insect-specific virus previously isolated in Japan, Indonesia, Texas, Mexico, Guatemala and Trinidad. The complete genome of one isolate was sequenced, as were the envelope protein genes of eight other isolates. Phylogenetic analysis revealed that CxFV isolates from the United States (Iowa and Texas) are more closely related to CxFV isolates from Asia than those from Mexico, Guatemala, and Trinidad.

© 2009 Entomological Society of America
Bradley J. Blitvich, Ming Lin, Karin S. Dorman, Victor Soto, Einat Hovav, Bradley J. Tucker, Molly Staley, Kenneth B. Platt, and Lyric C. Bartholomay "Genomic Sequence and Phylogenetic Analysis of Culex Flavivirus, an Insect-Specific Flavivirus, Isolated From Culex pipiens (Diptera: Culicidae) in Iowa," Journal of Medical Entomology 46(4), 934-941, (1 July 2009). https://doi.org/10.1603/033.046.0428
Received: 27 January 2009; Accepted: 14 April 2009; Published: 1 July 2009
JOURNAL ARTICLE
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KEYWORDS
Culex flavivirus
flavivirus
insect
mosquito
surveillance
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