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1 May 2014 A Fresh Liver Agar Substrate for Rearing Small Numbers of Forensically Important Blow Flies (Diptera: Calliphoridae)
Susan V. Gruner, Daniel H. Slone
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Abstract

Forensically important calliphorids can be reared on a mixture of beef liver and agar. Small pieces of meat, especially fresh or frozen beef liver, will desiccate in 2–6 h, but this simple-to-make feeding substrate remains moist for at least 12 h at 25 and 30°C without desiccation, even in small (5 g) amounts. We determined the survivorship of small numbers of Chrysomya megacephala (F.) (first-instar larvae to adult eclosion) raised on 5 g of liver agar and fresh beef liver. We found that all larvae raised on 5 g of liver died due to desiccation, but survivorship on 5 g of liver agar was equivalent to that on larger (50 g) pieces of either liver agar or beef liver.

© 2014 Entomological Society of America
Susan V. Gruner and Daniel H. Slone "A Fresh Liver Agar Substrate for Rearing Small Numbers of Forensically Important Blow Flies (Diptera: Calliphoridae)," Journal of Medical Entomology 51(3), 713-715, (1 May 2014). https://doi.org/10.1603/ME13070
Received: 8 April 2013; Accepted: 1 February 2014; Published: 1 May 2014
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KEYWORDS
Chrysomya megacephala
forensic entomology
rearing medium
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