Previous studies suggested the presence of species complex in the so-called Simulium asakoae Takaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present in Thailand, we performed a detailed morphological identification of S. asakoae and compared its DNA barcodes with the morphospecies S. asakoae from Myanmar and the typical S. asakoae from Malaysia. Phylogenetic analysis revealed the Thai materials analyzed in this study were indeed genetically similar with those from Myanmar and Malaysia, though genetic distances 0–2.27% were observed. We tentatively regard this divergence as intraspecific variation, and the automatic barcode gap discovery analysis further supports them as a single species.
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25 April 2020
DNA Barcoding of Simulium asakoae (Diptera: Simuliidae) From Northern Thailand
Van Lun Low,
Wichai Srisuka,
Atiporn Saeung,
Tiong Kai Tan,
Zubaidah Ya'cob,
Yze Shiuan Yeong,
Hiroyuki Takaoka
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Journal of Medical Entomology
Vol. 57 • No. 5
September 2020
Vol. 57 • No. 5
September 2020
black fly
COI gene
DNA barcode
Thailand
vector